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Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using ST14 Polyclonal antibody (27176-1-AP)

    WB analysis of MCF-7 using 27176-1-AP (same clone as 27176-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 27176-1-AP (ST14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27176-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using ST14 Polyclonal antibody (27176-1-AP)

    WB analysis of MCF-7 using 27176-1-AP (same clone as 27176-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 27176-1-AP (ST14 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27176-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using ST14 Polyclonal antibody (27176-1-AP)

    IHC staining of human colon using 27176-1-AP (same clone as 27176-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27176-1-AP (ST14 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 27176-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using ST14 Polyclonal antibody (27176-1-AP)

    IHC staining of human colon using 27176-1-AP (same clone as 27176-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27176-1-AP (ST14 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 27176-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using ST14 Polyclonal antibody (27176-1-AP)

    IHC staining of human stomach using 27176-1-AP (same clone as 27176-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 27176-1-AP (ST14 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 27176-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using ST14 Polyclonal antibody (27176-1-AP)

    IHC staining of human stomach using 27176-1-AP (same clone as 27176-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 27176-1-AP (ST14 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 27176-1-PBS in a different storage buffer formulation.

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ST14 Polyclonal antibody, PBS Only

ST14 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 27176-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27176-1-PBS targets ST14 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ST14 fusion protein Ag26007 相同性解析による交差性が予測される生物種
Full Name suppression of tumorigenicity 14 (colon carcinoma)
Calculated molecular weight 46 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC030532
Gene Symbol ST14
Gene ID (NCBI) 6768
RRIDAB_2880788
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y5Y6
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Suppression of tumorigenicity 14(ST14), is an epithelial-derived, integral membrane serine protease. ST14 forms a complex with the Kunitz-type serine protease inhibitor, HAI-1, and is found to be activated by sphingosine 1-phosphate. ST14 can cleave and activate hepatocyte growth factor/scattering factor, and uPA, which suggest the function of this protease as an epithelial membrane activator for other proteases and latent growth factors.