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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IL-1RL1/ST2 Polyclonal antibody

IL-1RL1/ST2 Polyclonal Antibody for WB, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC, ELISA

Conjugate

Unconjugated

Cat no : 30194-1-AP

Synonyms

IL1RL1, IL-33R, IL-1RL1, FIT 1, EC:3.2.2.6

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IL-1RL1/ST2 Polyclonal antibody (30194-1-AP)

    WB analysis using 30194-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30194-1-AP (IL-1RL1/ST2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HEK-293 cells using IL-1RL1/ST2 Polyclonal antibody (30194-1-AP)

    FC experiment of HEK-293 using 30194-1-AP

    1x10^6 HEK-293 cells were surface stained with 0.4 ug IL-1RL1/ST2 Polyclonal antibody (30194-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2) (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2) (blue). Cells were not fixed.

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Tested Applications

Positive WB detected inHUVEC cells, human placenta tissue
Positive FC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30194-1-AP targets IL-1RL1/ST2 in WB, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name interleukin 1 receptor-like 1
Calculated molecular weight 63 kDa, 37 kDa, 30 kDa
Observed molecular weight65 kDa
GenBank accession numberBC030975
Gene symbol IL1RL1
Gene ID (NCBI) 9173
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ST2, also known as IL1RL1, is a member of the IL-1 superfamily and serves as the receptor for IL-33. It plays a major role in immune and inflammatory responses. ST2 is expressed by various types of immune cells including Th2 cells, mast cells, type 2 innate lymphoid cells, eosinophils, basophils, NK cells, and non-immune cells, including cardiomyocytes. Two forms of ST2 exist: a membrane-bound form and a soluble form (sST2). The membrane-bound form activates the MyD88/NF-κB signaling pathway to enhance mast cell, Th2, regulatory T cell (Treg), and innate lymphoid cell type 2 functions. sST2 acts as a decoy receptor. (PMID: 27055914; 23999434; 28484466)

Protocols

Product Specific Protocols
WB protocol for IL-1RL1/ST2 antibody 30194-1-APDownload protocol
FC protocol for IL-1RL1/ST2 antibody 30194-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols