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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IL-1RL1/ST2 Monoclonal antibody, PBS Only

IL-1RL1/ST2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

7A2A7

Cat no : 60112-1-PBS

Synonyms

IL1RL1, IL-1RL1, FIT 1, EC:3.2.2.6, DER4

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using IL-1RL1/ST2 Monoclonal antibody (60112-1-Ig)

    WB analysis using 60112-1-Ig (same clone as 60112-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60112-1-Ig (ST2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60112-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using IL-1RL1/ST2 Monoclonal antibody (60112-1-Ig)

    WB analysis of HEK-293 using 60112-1-Ig (same clone as 60112-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 60112-1-Ig (ST2 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60112-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of fetal human brain tissue using IL-1RL1/ST2 Monoclonal antibody (60112-1-Ig)

    WB analysis of fetal human brain using 60112-1-Ig (same clone as 60112-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60112-1-Ig (ST2 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60112-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SGC-7901 cells using IL-1RL1/ST2 Monoclonal antibody (60112-1-Ig)

    WB analysis of SGC-7901 using 60112-1-Ig (same clone as 60112-1-PBS)

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 60112-1-Ig (ST2 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60112-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig brain tissue using IL-1RL1/ST2 Monoclonal antibody (60112-1-Ig)

    WB analysis of pig brain using 60112-1-Ig (same clone as 60112-1-PBS)

    pig brain tissue were subjected to SDS PAGE followed by western blot with 60112-1-Ig (ST2 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60112-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using IL-1RL1/ST2 Monoclonal antibody (60112-1-Ig)

    WB analysis of mouse brain using 60112-1-Ig (same clone as 60112-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 60112-1-Ig (ST2 antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60112-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60112-1-PBS targets IL-1RL1/ST2 in WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IL-1RL1/ST2 fusion protein Ag2555 相同性解析による交差性が予測される生物種
Full Name interleukin 1 receptor-like 1
Calculated molecular weight 63 kDa, 37 kDa, 30 kDa
Observed molecular weight 63 kDa
GenBank accession numberBC030975
Gene symbol IL1RL1
Gene ID (NCBI) 9173
RRIDAB_2264785
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

ST2, also known as IL1RL1, is a member of the IL-1 superfamily and serves as the receptor for IL-33. It plays a major role in immune and inflammatory responses. ST2 is expressed by various types of immune cells including Th2 cells, mast cells, type 2 innate lymphoid cells, eosinophils, basophils, NK cells, and non-immune cells, including cardiomyocytes. Two forms of ST2 exist: a membrane-bound form and a soluble form (sST2). The membrane-bound form activates the MyD88/NF-κB signaling pathway to enhance mast cell, Th2, regulatory T cell (Treg), and innate lymphoid cell type 2 functions. sST2 acts as a decoy receptor. (PMID: 27055914; 23999434; 28484466)