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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using ST3GAL3 Polyclonal antibody (24925-1-AP)

    WB analysis of mouse liver using 24925-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 24925-1-AP (ST3GAL3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin tissue using ST3GAL3 Polyclonal antibody (24925-1-AP)

    IHC staining of human skin using 24925-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 24925-1-AP (ST3GAL3 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using ST3GAL3 Polyclonal antibody (24925-1-AP)

    IHC staining of human skin using 24925-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 24925-1-AP (ST3GAL3 Antibody) at dilution of 1:200 (under 40x lens).

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  • Featured Product
  • KD/KO Validated

ST3GAL3 Polyclonal antibody

ST3GAL3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24925-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Alpha 2,3 ST 3, Sialyltransferase 6, SIAT6, ST3Gal III, ST3GAL3, ST3GALII, ST3GalIII, ST3N

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse liver tissue
Positive IHC detected inhuman skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24925-1-AP targets ST3GAL3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ST3GAL3 fusion protein Ag20795 相同性解析による交差性が予測される生物種
Full Name ST3 beta-galactoside alpha-2,3-sialyltransferase 3
Calculated molecular weight 375 aa, 42 kDa
Observed molecular weight 44-55 kDa
GenBank accession numberBC050380
Gene Symbol ST3GAL3
Gene ID (NCBI) 6487
RRIDAB_2879799
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ11203
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ST3GAL3 antibody 24925-1-APDownload protocol
IHC protocol for ST3GAL3 antibody 24925-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

FEBS Lett

The sialyltransferase ST3Gal3 facilitates the receptivity of the uterine endometrium in vitro and in vivo.

Authors - Ming Yu
  • KD Validated
View Article