ST3GAL4 Polyclonal antibody
ST3GAL4 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 13546-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | HCT 116 cells, HeLa cells, HepG2 cells, MCF-7 cells |
| Positive IHC detected in | human testis tissue, human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IHC | See 3 publications below |
| IF | See 1 publications below |
Product Information
13546-1-AP targets ST3GAL4 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ST3GAL4 fusion protein Ag3665 相同性解析による交差性が予測される生物種 |
| Full Name | ST3 beta-galactoside alpha-2,3-sialyltransferase 4 |
| Calculated molecular weight | 333 aa, 38 kDa |
| Observed molecular weight | 36 kDa |
| GenBank accession number | BC010645 |
| Gene symbol | ST3GAL4 |
| Gene ID (NCBI) | 6484 |
| RRID | AB_10597402 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ST3GAL4, also known as CGS23, NANTA3 and SIAT4C, is a member of the glycosyltransferase 29 family. ST3GAL4 protein exists in multiple splice variants and is frequently modified by glycosylation. ST3GAL4 is expressed uniformly in tissues and organs during all stages of development, and the subcellular localization is currently not clear.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ST3GAL4 antibody 13546-1-AP | Download protocol |
| IHC protocol for ST3GAL4 antibody 13546-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Oncol ST3Gal IV Mediates the Growth and Proliferation of Cervical Cancer Cells In Vitro and In Vivo Via the Notch/p21/CDKs Pathway. | ||
Sci Rep Five hypoxia and immunity related genes as potential biomarkers for the prognosis of osteosarcoma. | ||
FEBS Lett The sialyltransferase ST3Gal3 facilitates the receptivity of the uterine endometrium in vitro and in vivo. | ||
Glycobiology Sialylation-dependent interaction between PD-L1 and CD169 promotes monocytes adhesion to endothelial cells | ||
Elife RANK+TLR2+ myeloid subpopulation converts autoimmune to joint destruction in rheumatoid arthritis |
