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KD/KO Validated

ST3GAL5 Polyclonal antibody

Name: ST3GAL5 Polyclonal antibody
Brand: Proteintech
SKU: 14614-1-AP

ST3GAL5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 14614-1-AP

Synonyms

ST3GAL5, ST3GalV, UNQ2510/PRO5998, CMP-NeuAc:lactosylceramide alpha-2,3-sialyltransferase, EC:2.4.3.9, Lactosylceramide alpha-2,3-sialyltransferase, SIAT9, SIATGM3S, ST3Gal V

Validation Data Gallery

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AllWBIHC

WB analysis of HEK-293 using 14614-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 14614-1-AP (ST3GAL5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 14614-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 14614-1-AP (ST3GAL5 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14614-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14614-1-AP (ST3GAL5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 14614-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 14614-1-AP (ST3GAL5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 14614-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 14614-1-AP (ST3GAL5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of mouse skeletal muscle using 14614-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14614-1-AP (ST3GAL5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skeletal muscle using 14614-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14614-1-AP (ST3GAL5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skeletal muscle using 14614-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 14614-1-AP (ST3GAL5 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skeletal muscle using 14614-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 14614-1-AP (ST3GAL5 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human skin using 14614-1-AP
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 14614-1-AP (ST3GAL5 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human skin using 14614-1-AP
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 14614-1-AP (ST3GAL5 Antibody) at dilution of 1:200 (under 40x lens).

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Cat No. 14614-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HEK-293 cells, Jurkat cells, HeLa cells, human testis tissue, human placenta tissue
Positive IHC detected in	mouse skeletal muscle tissue, human skeletal muscle tissue, human skin tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below
IHC	See 3 publications below
IF	See 1 publications below

Product Information

14614-1-AP targets ST3GAL5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ST3GAL5 fusion protein Ag6207 相同性解析による交差性が予測される生物種
Full Name	ST3 beta-galactoside alpha-2,3-sialyltransferase 5
Calculated molecular weight	48 kDa
Observed molecular weight	45-50 kDa
GenBank accession number	BC065936
Gene symbol	ST3GAL5
Gene ID (NCBI)	8869
RRID	AB_2194414
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ST3GAL5 is also named as SIAT9, GM3 synthase, ST3Gal V, Sialyltransferase 9 and belongs to the glycosyltransferase 29 family. ST3GAL5 transfers the sialyl group (N-acetyl-alpha-neuraminyl or NeuAc) from CMP-NeuAc to the non-reducing terminal galactose of glycosphingolipids forming gangliosides (PMID:9822625, PMID:16934889). SIAT9 is mainly involved in the biosynthesis of ganglioside GM3 but can also use different glycolipids as substrate acceptors such as D-galactosylceramide (GalCer), asialo-GM2 (GA2) and asialo-GM1 (GA1) (PMID:16934889). ST3GAL5 is highly expressed in brain, skeletal muscle, placenta, and testis. And mRNA of ST3GAL5 is widely distributed in human brain, but slightly elevated expression was observed in the cerebral cortex, temporal lobe, and putamen (PMID:9822625). ST3GAL5 has 3 isoforms with the molecular mass of 45, 46, and 48 kDa. Sometimes the band of 70 kDa can also be observed, which may be a glycosylated form of ST3GAL5 (PMID: 34943806, PMID: 26458842).

Protocols

Product Specific Protocols
WB protocol for ST3GAL5 antibody 14614-1-AP	Download protocol
IHC protocol for ST3GAL5 antibody 14614-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	Front Immunol Identification of ST3GAL5 as a prognostic biomarker correlating with CD8+ T cell exhaustion in clear cell renal cell carcinoma Authors - Jiakuan Liu View Article
human	WB	Front Cell Dev Biol PRC2-dependent regulation of ganglioside expression during dedifferentiation contributes to the proliferation and migration of vascular smooth muscle cells Authors - Norihiko Sasaki View Article
human	WB	iScience C-Myc-induced hypersialylation of small cell lung cancer facilitates pro-tumoral phenotypes of macrophages Authors - Lin Tian View Article
human,mouse	WB,IHC,IF	Mol Oncol Exosomes secreted by ST3GAL5high cancer cells promote peritoneal dissemination by establishing a pre-metastatic microenvironment Authors - Misato Horie KO Validated View Article
human	WB,IHC	Int Immunopharmacol Identification and validation of sialyltransferase ST3Gal5 in bladder cancer through bioinformatics and experimental analysis Authors - Yuli Jian View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ST3GAL5 Polyclonal antibody

ST3GAL5 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis of HEK-293 using 14614-1-AP

WB analysis of Jurkat using 14614-1-AP

WB analysis of HeLa using 14614-1-AP

WB analysis of human testis using 14614-1-AP

WB analysis of human placenta using 14614-1-AP

IHC staining of mouse skeletal muscle using 14614-1-AP

IHC staining of mouse skeletal muscle using 14614-1-AP

IHC staining of human skeletal muscle using 14614-1-AP

IHC staining of human skeletal muscle using 14614-1-AP

IHC staining of human skin using 14614-1-AP

IHC staining of human skin using 14614-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Identification of ST3GAL5 as a prognostic biomarker correlating with CD8+ T cell exhaustion in clear cell renal cell carcinoma

PRC2-dependent regulation of ganglioside expression during dedifferentiation contributes to the proliferation and migration of vascular smooth muscle cells

C-Myc-induced hypersialylation of small cell lung cancer facilitates pro-tumoral phenotypes of macrophages

Exosomes secreted by ST3GAL5high cancer cells promote peritoneal dissemination by establishing a pre-metastatic microenvironment

Identification and validation of sialyltransferase ST3Gal5 in bladder cancer through bioinformatics and experimental analysis