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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ST3GAL5 Polyclonal antibody

ST3GAL5 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30120-1-AP

Synonyms

Ganglioside GM3 synthase, Sialyltransferase 9, SIAT9, SIATGM3S, ST3Gal V, ST3GAL5, ST3GalV

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ST3GAL5 Polyclonal antibody (30120-1-AP)

    WB analysis using 30120-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30120-1-AP (ST3GAL5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using ST3GAL5 Polyclonal antibody (30120-1-AP)

    IF Staining of U2OS using 30120-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using ST3GAL5 antibody (30120-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using ST3GAL5 Polyclonal antibody (30120-1-AP)

    IF Staining of U2OS using 30120-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using ST3GAL5 antibody (30120-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, human placenta tissue, mouse testis tissue
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30120-1-AP targets ST3GAL5 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ST3GAL5 fusion protein Ag32461 相同性解析による交差性が予測される生物種
Full Name ST3 beta-galactoside alpha-2,3-sialyltransferase 5
Calculated molecular weight 48 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC065936
Gene symbol ST3GAL5
Gene ID (NCBI) 8869
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ST3GAL5 is also named as SIAT9, GM3 synthase, ST3Gal V, Sialyltransferase 9 and belongs to the glycosyltransferase 29 family. ST3GAL5 transfers the sialyl group (N-acetyl-alpha-neuraminyl or NeuAc) from CMP-NeuAc to the non-reducing terminal galactose of glycosphingolipids forming gangliosides (PMID:9822625, PMID:16934889). SIAT9 is mainly involved in the biosynthesis of ganglioside GM3 but can also use different glycolipids as substrate acceptors such as D-galactosylceramide (GalCer), asialo-GM2 (GA2) and asialo-GM1 (GA1) (PMID:16934889). ST3GAL5 is high expression in brain, skeletal muscle, placenta, and testis. And mRNA of ST3GAL5 is widely distributed in human brain, but slightly elevated expression was observed in the cerebral cortex, temporal lobe, and putamen (PMID:9822625). This antibody recognizes the glycosylated form of ST3GAL5 (PMID: 34943806, PMID: 26458842).

Protocols

Product Specific Protocols
WB protocol for ST3GAL5 antibody 30120-1-APDownload protocol
IF protocol for ST3GAL5 antibody 30120-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols