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Validation Data Gallery

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  • Western Blot (WB) analysis of TF-1 cells using ST6GAL1 Monoclonal antibody (68633-1-Ig)

    WB analysis of TF-1 using 68633-1-Ig

    TF-1 cells were subjected to SDS PAGE followed by western blot with 68633-1-Ig (ST6GAL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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ST6GAL1 Monoclonal antibody

ST6GAL1 Monoclonal Antibody for WB, ELISA
Cat No. 68633-1-Ig
Clone No.1F11G5

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

WB, ELISA

Synonyms

CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1, CD75, Beta-galactoside alpha-2,6-sialyltransferase 1, B-cell antigen CD75, Alpha 2,6-ST 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inTF-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68633-1-Ig targets ST6GAL1 in WB, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen ST6GAL1 fusion protein Ag6181 相同性解析による交差性が予測される生物種
Full Name ST6 beta-galactosamide alpha-2,6-sialyltranferase 1
Calculated molecular weight 47 kDa
Observed molecular weight 43-45 kDa, 50-70 kDa
GenBank accession numberBC040009
Gene Symbol ST6GAL1
Gene ID (NCBI) 6480
RRIDAB_3085324
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP15907
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ST6GAL1 (β-galactoside α-2-6 sialyl transferase1; also known as ST6N or CD75) is a sialyltransferase mediating the glycosylation of proteins and lipids to form functionally important glycoproteins and glycolipids in the Golgi compartment. It is principally expressed in liver, placenta and skeletal muscle. ST6GAL1 undergoes proteolytic process to generate soluble form from membrane form. Western blot analysis of human liver using this antibody detects both isoforms between 43-50 kDa. Higher molecular weight of bands around 50-70 kDa can also be observed with unknown reason. (PMID: 15049997)

Protocols

Product Specific Protocols
WB protocol for ST6GAL1 antibody 68633-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols