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Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of HeLa cells using ST6GALNAC4 Polyclonal antibody (15654-1-AP)

    IP experiment of HeLa using 15654-1-AP

    IP result of anti-ST6GALNAC4 (IP:15654-1-AP, 4ug; Detection:15654-1-AP 1:300) with HeLa cells lysate 980 ug.

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ST6GALNAC4 Polyclonal antibody

ST6GALNAC4 Polyclonal Antibody for IP, ELISA
Cat No. 15654-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IP, ELISA

Synonyms

Sialyltransferase 3C, Sialyltransferase 7D, SIAT3 C, SIAT3C, SIAT7 D, SIAT7D, ST6GalNAc IV, ST6GALNAC4, ST6GALNACIV

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15654-1-AP targets ST6GALNAC4 in IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ST6GALNAC4 fusion protein Ag7995 相同性解析による交差性が予測される生物種
Full Name ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 4
Calculated molecular weight 34 kDa
Observed molecular weight35 kDa
GenBank accession numberBC036705
Gene Symbol ST6GALNAC4
Gene ID (NCBI) 27090
RRIDAB_3669218
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H4F1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3-N-acetyl-galactosaminide alpha-2,6-sialyltransferase (also known as ST6GALNAC4) is a member of the sialyltransferases, which catalyzes the transfer of sialic acid from cytidine monosphosphate (CMP)-sialic acid to galactose-containing substrates. ST6GALNAC4 may be induced abnormal TGFBR2 glycosylation, resulting in the higher protein levels of TGFBR2 and TGF pathway increased activation (PMID: 37381011, 37034303). ST6GALNAC4 was reported to influence patient prognosis though subverting immunosurveillance in Chronic lymphocytic leukemia.

Protocols

Product Specific Protocols
IP protocol for ST6GALNAC4 antibody 15654-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols