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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

STAG2 Polyclonal antibody

STAG2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 19837-1-AP

Synonyms

Stromal antigen 2, SA2, SA 2, Cohesin subunit SA-2, Cohesin subunit SA 2

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using STAG2 Polyclonal antibody (19837-1-AP)

    WB analysis of Jurkat using 19837-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 19837-1-AP (STAG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using STAG2 Polyclonal antibody (19837-1-AP)

    WB analysis of MCF-7 using 19837-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 19837-1-AP (STAG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using STAG2 Polyclonal antibody (19837-1-AP)

    WB analysis of NIH/3T3 using 19837-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 19837-1-AP (STAG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using STAG2 Polyclonal antibody (19837-1-AP)

    WB analysis of HeLa using 19837-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 19837-1-AP (STAG2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using STAG2 Polyclonal antibody (19837-1-AP)

    WB analysis of K-562 using 19837-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 19837-1-AP (STAG2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using STAG2 Polyclonal antibody (19837-1-AP)

    IP experiment of MCF-7 using 19837-1-AP

    IP result of anti-STAG2 (IP:19837-1-AP, 4ug; Detection:19837-1-AP 1:3000) with MCF-7 cells lysate 2120 ug.

  • Immunoprecipitation (IP) experiment of U-251 cells using STAG2 Polyclonal antibody (19837-1-AP)

    IP experiment of U-251 using 19837-1-AP

    IP result of anti-STAG2 (IP:19837-1-AP, 4ug; Detection:19837-1-AP 1:3000) with U-251 cells lysate 3320 ug.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using STAG2 Polyclonal antibody (19837-1-AP)

    IHC staining of human cervical cancer using 19837-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 19837-1-AP (STAG2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using STAG2 Polyclonal antibody (19837-1-AP)

    IHC staining of human cervical cancer using 19837-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 19837-1-AP (STAG2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using STAG2 Polyclonal antibody (19837-1-AP)

    IF Staining of HEK-293 using 19837-1-AP

    Immunofluorescent analysis of 293 cells, using STAG2 antibody 19837-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using STAG2 Polyclonal antibody (19837-1-AP)

    IF Staining of HEK-293 using 19837-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using STAG2 antibody (19837-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inJurkat cells, HeLa cells, K-562 cells, MCF-7 cells, NIH/3T3 cells
Positive IP detected inU-251 cells, MCF-7 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

19837-1-AP targets STAG2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STAG2 fusion protein Ag13825 相同性解析による交差性が予測される生物種
Full Name stromal antigen 2
Calculated molecular weight 1231 aa, 141 kDa
Observed molecular weight 130-141 kDa
GenBank accession numberBC001765
Gene symbol STAG2
Gene ID (NCBI) 10735
RRIDAB_10666847
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

STAG2, also named as SA2, is a component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex may play a role in spindle pole assembly during mitosis. Mutations in STAG2 appear to be a first step in the transformation of a normal cell into a cancer cell.

Protocols

Product Specific Protocols
WB protocol for STAG2 antibody 19837-1-APDownload protocol
IHC protocol for STAG2 antibody 19837-1-APDownload protocol
IF protocol for STAG2 antibody 19837-1-APDownload protocol
IP protocol for STAG2 antibody 19837-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Commun (Lond)

Squalene epoxidase promotes colorectal cancer cell proliferation through accumulating calcitriol and activating CYP24A1-mediated MAPK signaling.

Authors - Luwei He
View Article