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STAM2 Polyclonal antibody

STAM2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13009-1-AP

Synonyms

STAM2A, STAM-2, STAM 2, Hrs-binding protein, Hrs binding protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STAM2 Polyclonal antibody (13009-1-AP)

    WB analysis using 13009-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13009-1-AP (STAM2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using STAM2 Polyclonal antibody (13009-1-AP)

    WB analysis of NIH/3T3 using 13009-1-AP

    WB result of STAM2 antibody (13009-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STAM2 transfected NIH/3T3 cells.

  • Western Blot (WB) analysis of mouse brain tissue using STAM2 Polyclonal antibody (13009-1-AP)

    WB analysis of mouse brain using 13009-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13009-1-AP (STAM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using STAM2 Polyclonal antibody (13009-1-AP)

    WB analysis using 13009-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13009-1-AP (STAM2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using STAM2 Polyclonal antibody (13009-1-AP)

    IHC staining of mouse heart using 13009-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13009-1-AP (STAM2 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using STAM2 Polyclonal antibody (13009-1-AP)

    IHC staining of mouse kidney using 13009-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13009-1-AP (STAM2 antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using STAM2 Polyclonal antibody (13009-1-AP)

    IF Staining of HeLa using 13009-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using STAM2 antibody (13009-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, HepG2 cells
Positive IHC detected inmouse heart tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13009-1-AP targets STAM2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STAM2 fusion protein Ag3698 相同性解析による交差性が予測される生物種
Full Name signal transducing adaptor molecule (SH3 domain and ITAM motif) 2
Calculated molecular weight 58 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC028740
Gene symbol STAM2
Gene ID (NCBI) 10254
RRIDAB_2197497
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for STAM2 antibody 13009-1-APDownload protocol
IHC protocol for STAM2 antibody 13009-1-APDownload protocol
IF protocol for STAM2 antibody 13009-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols