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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

STAMBPL1 Polyclonal antibody

STAMBPL1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27315-1-AP

Synonyms

ALMalpha, AMSH FP, AMSH like protease, AMSH LP, AMSHLP, KIAA1373, STAM binding protein like 1, STAMBPL1

Validation Data Gallery

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  • Western Blot (WB) analysis of human testis tissue using STAMBPL1 Polyclonal antibody (27315-1-AP)

    WB analysis of human testis using 27315-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 27315-1-AP (STAMBPL1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using STAMBPL1 Polyclonal antibody (27315-1-AP)

    WB analysis using 27315-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27315-1-AP (STAMBPL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using STAMBPL1 Polyclonal antibody (27315-1-AP)

    WB analysis of A549 using 27315-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 27315-1-AP (STAMBPL1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using STAMBPL1 Polyclonal antibody (27315-1-AP)

    WB analysis of HeLa using 27315-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 27315-1-AP (STAMBPL1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using STAMBPL1 Polyclonal antibody (27315-1-AP)

    WB analysis of MCF-7 using 27315-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 27315-1-AP (STAMBPL1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using STAMBPL1 Polyclonal antibody (27315-1-AP)

    IHC staining of human liver using 27315-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27315-1-AP (STAMBPL1 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using STAMBPL1 Polyclonal antibody (27315-1-AP)

    IHC staining of human liver using 27315-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27315-1-AP (STAMBPL1 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using STAMBPL1 Polyclonal antibody (27315-1-AP)

    IHC staining of human colon using 27315-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27315-1-AP (STAMBPL1 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using STAMBPL1 Polyclonal antibody (27315-1-AP)

    IHC staining of human colon using 27315-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27315-1-AP (STAMBPL1 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

Positive WB detected inA549 cells, HeLa cells, MCF-7 cells, human testis tissue, Jurkat cells
Positive IHC detected inhuman liver tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27315-1-AP targets STAMBPL1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STAMBPL1 fusion protein Ag26308 相同性解析による交差性が予測される生物種
Full Name STAM binding protein-like 1
Observed molecular weight 50 kDa
GenBank accession numberBC010846
Gene symbol STAMBPL1
Gene ID (NCBI) 57559
RRIDAB_2880840
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for STAMBPL1 antibody 27315-1-APDownload protocol
IHC protocol for STAMBPL1 antibody 27315-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Mol Carcinog

STAMBPL1, transcriptionally regulated by SREBP1, promotes malignant behaviors of hepatocellular carcinoma cells via Wnt/β-catenin signaling pathway

Authors - Junyi Jin
View Article
humanWB

Transl Oncol

STAMBPL1 promotes the progression of lung adenocarcinoma by inhibiting DHRS2 expression

Authors - Xiang Yang
View Article