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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STAR Recombinant antibody (80751-1-RR)

    WB analysis using 80751-1-RR (same clone as 80751-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80751-1-RR (STAR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80751-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using STAR Recombinant antibody (80751-1-RR)

    WB analysis using 80751-1-RR (same clone as 80751-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80751-1-RR (STAR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80751-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using STAR Recombinant antibody (80751-1-RR)

    WB analysis using 80751-1-RR (same clone as 80751-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80751-1-RR (STAR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80751-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using STAR Recombinant antibody (80751-1-RR)

    IHC staining of mouse testis using 80751-1-RR (same clone as 80751-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 80751-1-RR (STAR antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80751-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using STAR Recombinant antibody (80751-1-RR)

    IHC staining of mouse testis using 80751-1-RR (same clone as 80751-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 80751-1-RR (STAR antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80751-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using STAR Recombinant antibody (80751-1-RR)

    IF Staining of mouse testis using 80751-1-RR (same clone as 80751-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using STAR antibody (80751-1-RR, Clone: 5M6 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80751-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80751-1-RR

    Affinity and Kinetic Characterization of 80751-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80751-1-RR against Human STAR were performed. The affinity constant is 0.676 nM.

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STAR Recombinant antibody, PBS Only

STAR Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 80751-1-PBS
Clone No.5M6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

START domain-containing protein 1, STARD1, 5M6

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80751-1-PBS targets STAR in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen STAR fusion protein Ag2862 相同性解析による交差性が予測される生物種
Full Name steroidogenic acute regulatory protein
Calculated molecular weight 285 aa, 32 kDa
Observed molecular weight32 kDa
GenBank accession numberBC010550
Gene Symbol STAR
Gene ID (NCBI) 6770
RRIDAB_2918910
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49675
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Steroidogenic acute regulatory protein (StAR) is a key player in acute regulation of hormone-induced steroidogenesis, by facilitating cholesterol transport from cellular stores to inner mitochondria membrane where the cholesterol was conversed to pregnenolone. StAR is prevalently expressed in mitochondria of steroid-producing adrenal and gonadal tissue.