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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

STAR Recombinant antibody, PBS Only

STAR Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat, pig, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5M6

Cat no : 80751-1-PBS

Synonyms

START domain-containing protein 1, STARD1, 5M6

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using STAR Recombinant antibody (80751-1-RR)

    WB analysis using 80751-1-RR (same clone as 80751-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80751-1-RR (STAR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80751-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using STAR Recombinant antibody (80751-1-RR)

    WB analysis using 80751-1-RR (same clone as 80751-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80751-1-RR (STAR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80751-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using STAR Recombinant antibody (80751-1-RR)

    WB analysis using 80751-1-RR (same clone as 80751-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80751-1-RR (STAR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80751-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using STAR Recombinant antibody (80751-1-RR)

    IHC staining of mouse testis using 80751-1-RR (same clone as 80751-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 80751-1-RR (STAR antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80751-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using STAR Recombinant antibody (80751-1-RR)

    IHC staining of mouse testis using 80751-1-RR (same clone as 80751-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 80751-1-RR (STAR antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80751-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using STAR Recombinant antibody (80751-1-RR)

    IF Staining of mouse testis using 80751-1-RR (same clone as 80751-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using STAR antibody (80751-1-RR, Clone: 5M6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80751-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80751-1-PBS

    Affinity and Kinetic Characterization of 80751-1-PBS

    Biolayer interferometry (BLl) kinetic assays of 80751-1-RR against Human STAR were performed. The affinity constant is 0.676 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

80751-1-PBS targets STAR in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, rat, pig, mouse samples.

Tested Reactivity Human, rat, pig, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen STAR fusion protein Ag2862 相同性解析による交差性が予測される生物種
Full Name steroidogenic acute regulatory protein
Calculated molecular weight 285 aa, 32 kDa
Observed molecular weight32 kDa
GenBank accession numberBC010550
Gene symbol STAR
Gene ID (NCBI) 6770
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Steroidogenic acute regulatory protein (StAR) is a key player in acute regulation of hormone-induced steroidogenesis, by facilitating cholesterol transport from cellular stores to inner mitochondria membrane where the cholesterol was conversed to pregnenolone. StAR is prevalently expressed in mitochondria of steroid-producing adrenal and gonadal tissue.