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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

STARD10 Monoclonal antibody

STARD10 Monoclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit, pig

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1B9A1

Cat no : 68554-1-Ig

Synonyms

Antigen NY CO 28, CGI 52, NY CO 28, PCTP L, PCTP like protein, PCTP2, SDCCAG28, STARD10

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STARD10 Monoclonal antibody (68554-1-Ig)

    WB analysis using 68554-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68554-1-Ig (STARD10 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using STARD10 Monoclonal antibody (68554-1-Ig)

    IP experiment of HepG2 using 68554-1-Ig

    IP result of anti-STARD10 (IP:68554-1-Ig, 4ug; Detection:68554-1-Ig 1:6000) with HepG2 cells lysate 1480 ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using STARD10 Monoclonal antibody (68554-1-Ig)

    IF Staining of HeLa using 68554-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using STARD10 antibody (68554-1-Ig, Clone: 1B9A1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inSK-BR-3 cells, MCF-7 cells, T-47D cells, LNCaP cells, rat liver tissue, mouse liver tissue, pig liver tissue, rabbit liver tissue, rabbit testis tissue
Positive IP detected inHepG2 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68554-1-Ig targets STARD10 in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

Tested Reactivity Human, mouse, rat, rabbit, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen STARD10 fusion protein Ag10932 相同性解析による交差性が予測される生物種
Full Name StAR-related lipid transfer (START) domain containing 10
Calculated molecular weight 291 aa, 33 kDa
Observed molecular weight35-40 kDa
GenBank accession numberBC007919
Gene symbol STARD10
Gene ID (NCBI) 10809
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

STARD10 (START domain-containing protein 10, also known as PCTP-like protein) is highly expressed in liver and responsible to transfer phosphatidylcholine. STARD10 was identified as a protein overexpressed in breast cancer that cooperates with the ErbB family of receptor tyrosine kinases in cellular transformation (PMID: 15911624). This antibody detected duplicate bands between 35-40 kDa (PMID: 15150109).

Protocols

Product Specific Protocols
WB protocol for STARD10 antibody 68554-1-IgDownload protocol
IF protocol for STARD10 antibody 68554-1-IgDownload protocol
IP protocol for STARD10 antibody 68554-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols