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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STAT1 Recombinant antibody (82016-1-RR)

    WB analysis using 82016-1-RR (same clone as 82016-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82016-1-RR (STAT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82016-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using STAT1 Recombinant antibody (82016-1-RR)

    WB analysis of HEK-293 using 82016-1-RR (same clone as 82016-1-PBS)

    WB result of STAT1 antibody (82016-1-RR; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STAT1 transfected HEK-293 cells. This data was developed using the same antibody clone with 82016-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of PC-3 cells using STAT1 Recombinant antibody (82016-1-RR)

    IP experiment of PC-3 using 82016-1-RR (same clone as 82016-1-PBS)

    IP result of anti-STAT1 (IP:82016-1-RR, 4ug; Detection:82016-1-RR 1:10000) with PC-3 cells lysate 2000 ug. This data was developed using the same antibody clone with 82016-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using STAT1 Recombinant antibody (82016-1-RR)

    IHC staining of human ovary tumor using 82016-1-RR (same clone as 82016-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 82016-1-RR (STAT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82016-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using STAT1 Recombinant antibody (82016-1-RR)

    IHC staining of human ovary tumor using 82016-1-RR (same clone as 82016-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 82016-1-RR (STAT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82016-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat colon tissue using STAT1 Recombinant antibody (82016-1-RR)

    IHC staining of rat colon using 82016-1-RR (same clone as 82016-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 82016-1-RR (STAT1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82016-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat spleen tissue using STAT1 Recombinant antibody (82016-1-RR)

    IHC staining of rat spleen using 82016-1-RR (same clone as 82016-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 82016-1-RR (STAT1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82016-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using STAT1 Recombinant antibody (82016-1-RR)

    IHC staining of mouse colon using 82016-1-RR (same clone as 82016-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 82016-1-RR (STAT1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82016-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using STAT1 Recombinant antibody (82016-1-RR)

    IHC staining of mouse spleen using 82016-1-RR (same clone as 82016-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 82016-1-RR (STAT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82016-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using STAT1 Recombinant antibody (82016-1-RR)

    IF Staining of HeLa using 82016-1-RR (same clone as 82016-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed IFN alpha treated HeLa cells using STAT1 antibody (82016-1-RR, Clone: 2F16 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 82016-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82016-1-RR

    Affinity and Kinetic Characterization of 82016-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82016-1-RR against Human STAT1 were performed. The affinity constant is 0.501 nM.

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  • Featured Product
  • KD/KO Validated

STAT1 Recombinant antibody, PBS Only

STAT1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 82016-1-PBS
Clone No.2F16

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Transcription factor ISGF-3 components p91/p84, STAT91, Signal transducer and activator of transcription 1-alpha/beta, ISGF 3, 2F16

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82016-1-PBS targets STAT1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen STAT1 fusion protein Ag0199 相同性解析による交差性が予測される生物種
Full Name signal transducer and activator of transcription 1, 91kDa
Calculated molecular weight 83 kDa
Observed molecular weight 84 kDa
GenBank accession numberBC002704
Gene Symbol STAT1
Gene ID (NCBI) 6772
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP42224
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

STAT1 (signal transducers and activators of transcription 1) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. Various ligands, including interferons- and - , EGF, PDGF and IL-6, can activate STAT1. STAT1 protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens.