STAT2 Polyclonal antibody

Name: STAT2 Polyclonal antibody
Brand: Proteintech
SKU: 16674-1-AP

STAT2 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 16674-1-AP

Synonyms

STAT113, STAT 2, P113, ISGF3, ISGF 3

Validation Data Gallery

Filter:

AllWBIPIHC

WB analysis using 16674-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16674-1-AP (STAT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 16674-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 16674-1-AP (STAT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HT-1080 using 16674-1-AP
HT-1080 cells were subjected to SDS PAGE followed by western blot with 16674-1-AP (STAT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 16674-1-AP
IP result of anti-STAT2 (IP:16674-1-AP, 4ug; Detection:16674-1-AP 1:500) with HeLa cells lysate 2800ug.
IHC staining of human breast cancer using 16674-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 16674-1-AP (STAT2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human breast cancer using 16674-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 16674-1-AP (STAT2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human lung cancer using 16674-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 16674-1-AP (STAT2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung cancer using 16674-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 16674-1-AP (STAT2 antibody) at dilution of 1:50 (under 40x lens).

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Cat No. 16674-1-AP

Size/Concentration

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A431 cells, K-562 cells, HT-1080 cells, HeLa cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human breast cancer tissue, human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 20 publications below
IF	See 3 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

16674-1-AP targets STAT2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	STAT2 fusion protein Ag10168 相同性解析による交差性が予測される生物種
Full Name	signal transducer and activator of transcription 2, 113kDa
Calculated molecular weight	851 aa, 98 kDa
Observed molecular weight	113 kDa
GenBank accession number	BC051284
Gene symbol	STAT2
Gene ID (NCBI)	6773
RRID	AB_10644445
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

STAT2, also named as p113, belongs to the transcription factor STAT family. It is a signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN stimulated genes, which drive the cell in an antiviral state. It also interacts with CRSP2, CRSP6, Simian virus 5 protein V, rabies virus phosphoprotein, IFNAR1 and IFNAR2. Its interaction with dengue virus NS5 inhibits the phosphorylation of STAT2, and, when all viral proteins are present (polyprotein), STAT2 is targeted for degradation. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human STAT2. The calculated molecular weight of STAT2 is 98 kDa, but phosphorylated STAT2 is about 100-113 kDa.

Protocols

Product Specific Protocols
WB protocol for STAT2 antibody 16674-1-AP	Download protocol
IHC protocol for STAT2 antibody 16674-1-AP	Download protocol
IP protocol for STAT2 antibody 16674-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	J Pharm Anal Fangchinoline induces antiviral response by suppressing STING degradation Authors - Jinyong Wang View Article
human	WB	Int J Mol Sci A 2-Benzylmalonate Derivative as STAT3 Inhibitor Suppresses Tumor Growth in Hepatocellular Carcinoma by Upregulating β-TrCP E3 Ubiquitin Ligase. Authors - Ting Peng View Article
human	WB	Oncotarget Dietary quercetin potentiates the antiproliferative effect of interferon-α in hepatocellular carcinoma cells through activation of JAK/STAT pathway signaling by inhibition of SHP2 phosphatase. Authors - Ighodaro Igbe View Article
pig	WB	Int J Biol Macromol Atractylodis macrocephalae polysaccharides protect against DSS-induced intestinal injury through a novel lncRNA ITSN1-OT1. Authors - Xin Zong View Article
human	WB, IP, IF	J Immunol Herpes Simplex Virus Type 2 Inhibits Type I IFN Signaling Mediated by the Novel E3 Ubiquitin Protein Ligase Activity of Viral Protein ICP22. Authors - Mudan Zhang View Article
human	WB,IF,CoIP	J Biol Chem African swine fever virus pB475L evades host antiviral innate immunity via targeting STAT2 to inhibit IFN-I signaling Authors - Zhao Huang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

STAT2 Polyclonal antibody

STAT2 Polyclonal Antibody for WB, IHC, IP, ELISA

Validation Data Gallery

WB analysis using 16674-1-AP

WB analysis of K-562 using 16674-1-AP

WB analysis of HT-1080 using 16674-1-AP

IP experiment of HeLa using 16674-1-AP

IHC staining of human breast cancer using 16674-1-AP

IHC staining of human breast cancer using 16674-1-AP

IHC staining of human lung cancer using 16674-1-AP

IHC staining of human lung cancer using 16674-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Fangchinoline induces antiviral response by suppressing STING degradation

A 2-Benzylmalonate Derivative as STAT3 Inhibitor Suppresses Tumor Growth in Hepatocellular Carcinoma by Upregulating β-TrCP E3 Ubiquitin Ligase.

Dietary quercetin potentiates the antiproliferative effect of interferon-α in hepatocellular carcinoma cells through activation of JAK/STAT pathway signaling by inhibition of SHP2 phosphatase.

Atractylodis macrocephalae polysaccharides protect against DSS-induced intestinal injury through a novel lncRNA ITSN1-OT1.

Herpes Simplex Virus Type 2 Inhibits Type I IFN Signaling Mediated by the Novel E3 Ubiquitin Protein Ligase Activity of Viral Protein ICP22.

African swine fever virus pB475L evades host antiviral innate immunity via targeting STAT2 to inhibit IFN-I signaling