Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 16674-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 16674-1-AP (STAT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HT-1080 using 16674-1-AP
HT-1080 cells were subjected to SDS PAGE followed by western blot with 16674-1-AP (STAT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 16674-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 16674-1-AP (STAT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 16674-1-AP
IP result of anti-STAT2 (IP:16674-1-AP, 4ug; Detection:16674-1-AP 1:500) with HeLa cells lysate 2800ug.
IHC staining of human breast cancer using 16674-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 16674-1-AP (STAT2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human breast cancer using 16674-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer using 16674-1-AP (STAT2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human lung cancer using 16674-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 16674-1-AP (STAT2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung cancer using 16674-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 16674-1-AP (STAT2 antibody) at dilution of 1:50 (under 40x lens).

STAT2 Polyclonal antibody

STAT2 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 16674-1-AP

Publications(54)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

ISGF 3, ISGF3, P113, STAT 2, STAT113

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (54)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A431 cells, K-562 cells, HT-1080 cells, HeLa cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human breast cancer tissue, human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 42 publications below
IHC	See 1 publications below
IF	See 11 publications below
IP	See 3 publications below
CoIP	See 2 publications below

Product Information

16674-1-AP targets STAT2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse, pig, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag10168 Product name: Recombinant human STAT2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 503-851 aa of BC051284 Sequence: QFSSYVGRGLNSDQLSMLRNKLFGQNCRTEDPLLSWADFTKRESPPGKLPFWTWLDKILELVHDHLKDLWNDGRIMGFVSRSQERRLLKKTMSGTFLLRFSESSEGGITCSWVEHQDDDKVLIYSVQPYTKEVLQSLPLTEIIRHYQLLTEENIPENPLRFLYPRIPRDEAFGCYYQEKVNLQERRKYLKHRLIVVSNRQVDELQQPLELKPEPELESLELELGLVPEPELSLDLEPLLKAGLDLGPELESVLESTLEPVIEPTLCMVSQTVPEPDQGPVSQPVPEPDLPCDLRHLNTEPMEIFRNCVKIEEIMPNGDPLLAGQNTVDEVYVSRPSHFYTDGPLMPSDF 相同性解析による交差性が予測される生物種
Full Name	signal transducer and activator of transcription 2, 113kDa
Calculated molecular weight	851 aa, 98 kDa
Observed molecular weight	113 kDa
GenBank accession number	BC051284
Gene Symbol	STAT2
Gene ID (NCBI)	6773
RRID	AB_10644445
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P52630
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

STAT2, also named as p113, belongs to the transcription factor STAT family. It is a signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN stimulated genes, which drive the cell in an antiviral state. It also interacts with CRSP2, CRSP6, Simian virus 5 protein V, rabies virus phosphoprotein, IFNAR1 and IFNAR2. Its interaction with dengue virus NS5 inhibits the phosphorylation of STAT2, and, when all viral proteins are present (polyprotein), STAT2 is targeted for degradation. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human STAT2. The calculated molecular weight of STAT2 is 98 kDa, but phosphorylated STAT2 is about 100-113 kDa.

Protocols

Product Specific Protocols
IHC protocol for STAT2 antibody 16674-1-AP	Download protocol
IP protocol for STAT2 antibody 16674-1-AP	Download protocol
WB protocol for STAT2 antibody 16674-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Adv Mater LPS-Binding Hydrogel for TLR4-Mediated Microbiota-Immune Modulation. Authors - Jiali Chen View Article
pig,human	WB	Autophagy The Alpha-coronavirus E protein inhibits the JAK-STAT pathway signaling by triggering STAT2 degradation through OPTN- and NBR1-mediated selective autophagy Authors - Zhao Huang View Article
mouse,human	WB	Nat Commun Targeting LRBA triggers CTLA4 degradation and antitumor immunity for cancer immunotherapy. Authors - Xiaolu Ge View Article
mouse,human	WB,IP	J Clin Invest LC3-dependent intercellular transfer of phosphorylated STAT1/2 elicits CXCL9+ macrophages and enhances radiation-induced antitumor immunity Authors - Jun-Yan Li KD Validated View Article
mouse	WB	Adv Sci (Weinh) Electret-Inspired Charge-Injected Hydrogel for Scar-Free Healing of Bacterially Infected Burns Through Bioelectrical Stimulation and Immune Modulation Authors - Mujie Liu View Article
pig	WB	Adv Sci (Weinh) Mechanisms of Baicalin Alleviates Intestinal Inflammation: Role of M1 Macrophage Polarization and Lactobacillus amylovorus Authors - Shunfen Zhang View Article

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Validation Data Gallery

WB analysis using 16674-1-AP

WB analysis of HT-1080 using 16674-1-AP

WB analysis of K-562 using 16674-1-AP

IP experiment of HeLa using 16674-1-AP

IHC staining of human breast cancer using 16674-1-AP

IHC staining of human breast cancer using 16674-1-AP

IHC staining of human lung cancer using 16674-1-AP

IHC staining of human lung cancer using 16674-1-AP

STAT2 Polyclonal antibody

STAT2 Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

LPS-Binding Hydrogel for TLR4-Mediated Microbiota-Immune Modulation.

The Alpha-coronavirus E protein inhibits the JAK-STAT pathway signaling by triggering STAT2 degradation through OPTN- and NBR1-mediated selective autophagy

Targeting LRBA triggers CTLA4 degradation and antitumor immunity for cancer immunotherapy.

LC3-dependent intercellular transfer of phosphorylated STAT1/2 elicits CXCL9+ macrophages and enhances radiation-induced antitumor immunity

Electret-Inspired Charge-Injected Hydrogel for Scar-Free Healing of Bacterially Infected Burns Through Bioelectrical Stimulation and Immune Modulation

Mechanisms of Baicalin Alleviates Intestinal Inflammation: Role of M1 Macrophage Polarization and Lactobacillus amylovorus

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Validation Data Gallery

WB analysis using 16674-1-AP

WB analysis of HT-1080 using 16674-1-AP

WB analysis of K-562 using 16674-1-AP

IP experiment of HeLa using 16674-1-AP

IHC staining of human breast cancer using 16674-1-AP

IHC staining of human breast cancer using 16674-1-AP

IHC staining of human lung cancer using 16674-1-AP

IHC staining of human lung cancer using 16674-1-AP

STAT2 Polyclonal antibody

STAT2 Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

LPS-Binding Hydrogel for TLR4-Mediated Microbiota-Immune Modulation.

The Alpha-coronavirus E protein inhibits the JAK-STAT pathway signaling by triggering STAT2 degradation through OPTN- and NBR1-mediated selective autophagy

Targeting LRBA triggers CTLA4 degradation and antitumor immunity for cancer immunotherapy.

LC3-dependent intercellular transfer of phosphorylated STAT1/2 elicits CXCL9+ macrophages and enhances radiation-induced antitumor immunity

Electret-Inspired Charge-Injected Hydrogel for Scar-Free Healing of Bacterially Infected Burns Through Bioelectrical Stimulation and Immune Modulation

Mechanisms of Baicalin Alleviates Intestinal Inflammation: Role of M1 Macrophage Polarization and Lactobacillus amylovorus

Able™

Able^™