Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 10253-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 10253-2-AP
WB result of STAT3 antibody (10253-2-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STAT3 transfected A549 cells.
WB analysis using 10253-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis using 10253-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 10253-2-AP
A549 cells were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis using 10253-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:9000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 10253-2-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 10253-2-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 10253-2-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 10253-2-AP
A431 cells were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 10253-2-AP
A549 cells were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 10253-2-AP
IP result of anti-STAT3 (IP:10253-2-AP, 4ug; Detection:10253-2-AP 1:5000) with HeLa cells lysate 1280 ug.
IHC staining of human stomach cancer using 10253-2-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 10253-2-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10253-2-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 10253-2-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 10253-2-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 10253-2-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10253-2-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 10253-2-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 10253-2-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 10253-2-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 10253-2-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 10253-2-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 10253-2-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 10253-2-AP (STAT3 Antibody) at dilution of 1:50.
IHC staining of human breast cancer using 10253-2-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 10253-2-AP (STAT3 Antibody) at dilution of 1:50.
IF Staining of HeLa using 10253-2-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using STAT3 antibody (10253-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). DAPI (blue).
IF Staining of HeLa using 10253-2-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using STAT3 antibody (10253-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IF Staining of HeLa using 10253-2-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed IFN alpha treated HeLa cells using STAT3 antibody (10253-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).
IF Staining of HeLa using 10253-2-AP
Immunofluorescent analysis of HeLa cells using 10253-2-AP (STAT3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.
FC experiment of Ramos using 10253-2-AP
1X10^6 Ramos cells were intracellularly stained with 0.4 ug Anti-Human STAT3 (10253-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

STAT3 Polyclonal antibody

STAT3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 10253-2-AP

Publications(494)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA

Synonyms

STAT 3, HIES, APRF, Acute-phase response factor

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (494)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, A431 cells, HeLa cells, Jurkat cells, K-562 cells, MCF-7 cells, PC-12 cells, NIH/3T3 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human stomach cancer tissue, human colon cancer tissue, human cervical cancer tissue, mouse colon tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, IFN alpha treated HeLa cells
Positive FC (Intra) detected in	Ramos cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 25 publications below
WB	See 457 publications below
IHC	See 35 publications below
IF	See 34 publications below
IP	See 8 publications below
CoIP	See 8 publications below
ChIP	See 15 publications below
RIP	See 1 publications below

Product Information

10253-2-AP targets STAT3 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, zebrafish, bovine, marmoset
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	STAT3 fusion protein Ag0360 相同性解析による交差性が予測される生物種
Full Name	signal transducer and activator of transcription 3 (acute-phase response factor)
Calculated molecular weight	770 aa, 88 kDa
Observed molecular weight	88 kDa
GenBank accession number	BC000627
Gene Symbol	STAT3
Gene ID (NCBI)	6774
RRID	AB_2302876
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P40763
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human STAT3. STAT3 exists three isoforms and the molecular weight of each isoform respectively is 83 kDa, 87 kDa and 88 kDa.

Protocols

Product Specific Protocols
WB protocol for STAT3 antibody 10253-2-AP	Download protocol
IHC protocol for STAT3 antibody 10253-2-AP	Download protocol
IF protocol for STAT3 antibody 10253-2-AP	Download protocol
IP protocol for STAT3 antibody 10253-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cancer Cell Targeting the immune privilege of tumor-initiating cells to enhance cancer immunotherapy Authors - Chen Yang View Article
mouse	IHC	Nature Divergent transcriptional regulation of astrocyte reactivity across disorders. Authors - Joshua E Burda View Article
human,mouse	WB	Cell Metab Lactate modulates iron metabolism by binding soluble adenylyl cyclase Authors - Wei Liu View Article
mouse	WB	Brain Behav Immun l-Cysteine suppresses hypoxia-ischemia injury in neonatal mice by reducing glial activation, promoting autophagic flux and mediating synaptic modification via H2S formation. Authors - Danqing Xin View Article
mouse	WB	ACS Appl Mater Interfaces A Versatile Nanoplatform for Broad-Spectrum Immunotherapy by Reversing the Tumor Microenvironment Authors - Ao Chen View Article
mouse	WB	J Nanobiotechnology Nanocomposites based on nanoceria regulate the immune microenvironment for the treatment of polycystic ovary syndrome Authors - Sisi Yan View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 10253-2-AP

WB analysis of A549 using 10253-2-AP

WB analysis using 10253-2-AP

WB analysis using 10253-2-AP

WB analysis of A549 using 10253-2-AP

WB analysis using 10253-2-AP

WB analysis of K-562 using 10253-2-AP

WB analysis of MCF-7 using 10253-2-AP

WB analysis of Jurkat using 10253-2-AP

WB analysis of A431 using 10253-2-AP

WB analysis of A549 using 10253-2-AP

IP experiment of HeLa using 10253-2-AP

IHC staining of human stomach cancer using 10253-2-AP

IHC staining of human stomach cancer using 10253-2-AP

IHC staining of human colon cancer using 10253-2-AP

IHC staining of human breast cancer using 10253-2-AP

IHC staining of human cervical cancer using 10253-2-AP

IHC staining of human cervical cancer using 10253-2-AP

IHC staining of human colon cancer using 10253-2-AP

IHC staining of mouse colon using 10253-2-AP

IHC staining of mouse colon using 10253-2-AP

IHC staining of human cervical cancer using 10253-2-AP

IHC staining of human cervical cancer using 10253-2-AP

IHC staining of human cervical cancer using 10253-2-AP

IHC staining of human breast cancer using 10253-2-AP

IHC staining of human breast cancer using 10253-2-AP

IF Staining of HeLa using 10253-2-AP

IF Staining of HeLa using 10253-2-AP

IF Staining of HeLa using 10253-2-AP

IF Staining of HeLa using 10253-2-AP

FC experiment of Ramos using 10253-2-AP

STAT3 Polyclonal antibody

STAT3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Targeting the immune privilege of tumor-initiating cells to enhance cancer immunotherapy

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Lactate modulates iron metabolism by binding soluble adenylyl cyclase

l-Cysteine suppresses hypoxia-ischemia injury in neonatal mice by reducing glial activation, promoting autophagic flux and mediating synaptic modification via H2S formation.

A Versatile Nanoplatform for Broad-Spectrum Immunotherapy by Reversing the Tumor Microenvironment

Nanocomposites based on nanoceria regulate the immune microenvironment for the treatment of polycystic ovary syndrome