Various lysates were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

WB result of STAT3 antibody (10253-2-AP, 1:2000) with si-control and si-STAT3 transfected HeLa cells.

Various lysates were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

Various lysates were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:9000 incubated at room temperature for 1.5 hours.

mouse lung tissue were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Jurkat cells were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

A431 cells were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

A549 cells were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

mouse brain tissue were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

K-562 cells were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

mouse heart tissue were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

MCF7 cells were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

rat brain tissue were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10253-2-AP (STAT3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 10253-2-AP (STAT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 10253-2-AP (STAT3 Antibody) at dilution of 1:50.

Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 10253-2-AP (STAT3 Antibody) at dilution of 1:50.

IP Result of anti-STAT3 (IP:10253-2-AP, 3ug; Detection:10253-2-AP 1:2000) with HeLa cells lysate 3000ug.

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using STAT3 antibody (10253-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). DAPI (blue).

Immunofluorescent analysis of HeLa cells using 10253-2-AP (STAT3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

1X10^6 HeLa cells were stained with 0.2ug STAT3 antibody (10253-2-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.