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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STAT5A Monoclonal antibody (66459-1-Ig)

    WB analysis using 66459-1-Ig (same clone as 66459-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66459-1-Ig (STAT5A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66459-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-937 cells using STAT5A Monoclonal antibody (66459-1-Ig)

    WB analysis of U-937 using 66459-1-Ig (same clone as 66459-1-PBS)

    U-937 cells were subjected to SDS PAGE followed by western blot with 66459-1-Ig (STAT5A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66459-1-PBS in a different storage buffer formulation.

  • Sample test of MP50852-1

    Sample test of MP50852-1

    Cytometric bead array in cell lysate using MP50852-1, Phospho-STAT5A (Ser726) Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66459-1-PBS. Detection antibody: 60596-1-PBS. Cell lysate: Non-treated TF-1 and Calyculin A treated TF-1 (30μg/well). Non-related target Tubulin-beta Recombinant Matched Antibody Pair (MP00550-1) was served as control.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using STAT5A Monoclonal antibody (66459-1-Ig)

    IF Staining of HepG2 using 66459-1-Ig (same clone as 66459-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using STAT5A antibody (66459-1-Ig, Clone: 1F10F6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66459-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using STAT5A Monoclonal antibody (66459-1-Ig)

    IF Staining of HepG2 using 66459-1-Ig (same clone as 66459-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using STAT5A antibody (66459-1-Ig, Clone: 1F10F6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66459-1-PBS in a different storage buffer formulation.

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STAT5A Monoclonal antibody, PBS Only (Capture)

STAT5A Monoclonal Antibody for WB, IF/ICC, Indirect ELISA, Sample test
Cat No. 66459-1-PBS
Clone No.1F10F6

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, Indirect ELISA, Sample test

Synonyms

STAT5, MGF, 1F10F6

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66459-1-PBS targets STAT5A as part of a matched antibody pair:

MP50852-1: 66459-1-PBS capture and 60596-1-PBS detection (validated in N/A)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen STAT5A fusion protein Ag19546 相同性解析による交差性が予測される生物種
Full Name signal transducer and activator of transcription 5A
Calculated molecular weight 794 aa, 92 kDa
Observed molecular weight 95 kDa
GenBank accession numberBC027036
Gene Symbol STAT5A
Gene ID (NCBI) 6776
RRIDAB_2881828
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP42229
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

STAT5A, also named as STAT5, belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5A is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different GHs. Activation of STAT5A in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of human STAT5A is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of STAT5A in cells.