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  • Featured Product
  • KD/KO Validated

STAT6 Polyclonal antibody

STAT6 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 51073-1-AP

Synonyms

D12S1644, IL 4 STAT, STAT6, STAT6B, STAT6C

Validation Data Gallery

Applications:
  • WB analysis of NIH/3T3 using 51073-1-AP

    WB analysis of NIH/3T3 using 51073-1-AP

    WB result of STAT6 antibody (51073-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STAT6 transfected NIH/3T3 cells.

  • WB analysis of NIH/3T3 using 51073-1-AP

    WB analysis of NIH/3T3 using 51073-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 51073-1-AP (STAT6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis using 51073-1-AP

    WB analysis using 51073-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 51073-1-AP (STAT6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 51073-1-AP

    WB analysis of MCF-7 using 51073-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 51073-1-AP (STAT6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human spleen using 51073-1-AP

    IHC staining of human spleen using 51073-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 51073-1-AP (STAT6 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with STAT6 antibody included
  • IHC staining of human spleen using 51073-1-AP

    IHC staining of human spleen using 51073-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 51073-1-AP (STAT6 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with STAT6 antibody included
  • IHC staining of human colon cancer using 51073-1-AP

    IHC staining of human colon cancer using 51073-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 51073-1-AP (STAT6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STAT6 antibody included
  • IHC staining of mouse lung using 51073-1-AP

    IHC staining of mouse lung using 51073-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 51073-1-AP (STAT6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STAT6 antibody included
  • IHC staining of human lung using 51073-1-AP

    IHC staining of human lung using 51073-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 51073-1-AP (STAT6 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with STAT6 antibody included
  • IHC staining of human lung using 51073-1-AP

    IHC staining of human lung using 51073-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 51073-1-AP (STAT6 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with STAT6 antibody included
  • IHC staining of mouse lung using 51073-1-AP

    IHC staining of mouse lung using 51073-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 51073-1-AP (STAT6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STAT6 antibody included
  • IHC staining of mouse lung using 51073-1-AP

    IHC staining of mouse lung using 51073-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 51073-1-AP (STAT6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STAT6 antibody included

Tested Applications

Positive WB detected inHeLa cells, NIH/3T3 cells, MCF-7 cells, THP-1 cells, Jurkat cells, PC-12 cells
Positive IHC detected inhuman spleen tissue, human lung tissue, mouse lung tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy STAT6 Ready-To-Use IHC Kit. STAT6 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

51073-1-AP targets STAT6 in WB, RIP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name signal transducer and activator of transcription 6, interleukin-4 induced
Calculated molecular weight 94 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC075852
Gene symbol STAT6
Gene ID (NCBI) 6778
RRIDAB_2197244
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

STAT6, also named as Signal transducer and activator of transcription 6, is a 847 amino acid protein, which belongs to the transcription factor STAT family. STAT6 forms a homodimer or a heterodimer with a related family member. STAT6 translocates into the nucleus in response to phosphorylation. STAT6 is Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling. The antibody could identify about 100 kDa band, similar to the reported in the literature(PMID: 10961983).

Protocols

Product Specific Protocols
WB protocol for STAT6 antibody 51073-1-APDownload protocol
IHC protocol for STAT6 antibody 51073-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Proc Natl Acad Sci U S A

miR-182 targeting reprograms tumor-associated macrophages and limits breast cancer progression.

Authors - Chengxin Ma
View Article
human,mouseWB

Cell Rep

Macrophage autophagy deficiency-induced CEBPB accumulation alleviates atopic dermatitis via impairing M2 polarization

Authors - Yongcheng Zhu
View Article
humanIF

Front Cell Infect Microbiol

Ehrlichia chaffeensis Tandem Repeat Effector Targets Differentially Influence Infection.

Authors - Tian Luo
View Article
humanWB

Front Cell Dev Biol

A miR-129-5P/ARID3A Negative Feedback Loop Modulates Diffuse Large B Cell Lymphoma Progression and Immune Evasion Through Regulating the PD-1/PD-L1 Checkpoint

Authors - Weili Zheng
View Article
humanWB

Cells

Determination of WWOX Function in Modulating Cellular Pathways Activated by AP-2α and AP-2γ Transcription Factors in Bladder Cancer.

Authors - Damian Kołat
View Article
mouse,ratWB,IHC

Life Sci

Berberine regulates macrophage polarization through IL-4-STAT6 signaling pathway in Helicobacter pylori-induced chronic atrophic gastritis.

Authors - Tao Yang
View Article