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STAT6 Polyclonal antibody

Name: STAT6 Polyclonal antibody
Brand: Proteintech
SKU: 51073-1-AP

STAT6 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ChIP, RIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 51073-1-AP

Synonyms

D12S1644, IL 4 STAT, STAT6, STAT6B, STAT6C

Validation Data Gallery

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AllWBIHC

WB analysis of NIH/3T3 using 51073-1-AP
WB result of STAT6 antibody (51073-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STAT6 transfected NIH/3T3 cells.
WB analysis of NIH/3T3 using 51073-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 51073-1-AP (STAT6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis using 51073-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 51073-1-AP (STAT6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis using 51073-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 51073-1-AP (STAT6 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 51073-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 51073-1-AP (STAT6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IHC staining of human spleen using 51073-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 51073-1-AP (STAT6 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human spleen using 51073-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 51073-1-AP (STAT6 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human colon cancer using 51073-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 51073-1-AP (STAT6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse lung using 51073-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 51073-1-AP (STAT6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse lung using 51073-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 51073-1-AP (STAT6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse lung using 51073-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 51073-1-AP (STAT6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung using 51073-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 51073-1-AP (STAT6 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human lung using 51073-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 51073-1-AP (STAT6 antibody) at dilution of 1:50 (under 40x lens).

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Cat No. 51073-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, NIH/3T3 cells, MCF-7 cells, THP-1 cells, Jurkat cells, PC-12 cells
Positive IHC detected in	human spleen tissue, human lung tissue, mouse lung tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 22 publications below
IHC	See 1 publications below
IF	See 2 publications below
ChIP	See 1 publications below
RIP	See 1 publications below

Product Information

51073-1-AP targets STAT6 in WB, IHC, IF, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	signal transducer and activator of transcription 6, interleukin-4 induced
Calculated molecular weight	94 kDa
Observed molecular weight	100-110 kDa
GenBank accession number	BC075852
Gene symbol	STAT6
Gene ID (NCBI)	6778
RRID	AB_2197244
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

STAT6, also named as Signal transducer and activator of transcription 6, is a 847 amino acid protein, which belongs to the transcription factor STAT family. STAT6 forms a homodimer or a heterodimer with a related family member. STAT6 translocates into the nucleus in response to phosphorylation. STAT6 is Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling. The antibody could identify about 100 kDa band, similar to the reported in the literature(PMID: 10961983).

Protocols

Product Specific Protocols
WB protocol for STAT6 antibody 51073-1-AP	Download protocol
IHC protocol for STAT6 antibody 51073-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Proc Natl Acad Sci U S A miR-182 targeting reprograms tumor-associated macrophages and limits breast cancer progression. Authors - Chengxin Ma View Article
human,mouse	WB	Cell Rep Macrophage autophagy deficiency-induced CEBPB accumulation alleviates atopic dermatitis via impairing M2 polarization Authors - Yongcheng Zhu View Article
human	IF	Front Cell Infect Microbiol Ehrlichia chaffeensis Tandem Repeat Effector Targets Differentially Influence Infection. Authors - Tian Luo View Article
human	WB	Front Cell Dev Biol A miR-129-5P/ARID3A Negative Feedback Loop Modulates Diffuse Large B Cell Lymphoma Progression and Immune Evasion Through Regulating the PD-1/PD-L1 Checkpoint Authors - Weili Zheng View Article
human	WB	Cells Determination of WWOX Function in Modulating Cellular Pathways Activated by AP-2α and AP-2γ Transcription Factors in Bladder Cancer. Authors - Damian Kołat View Article
mouse,rat	WB,IHC	Life Sci Berberine regulates macrophage polarization through IL-4-STAT6 signaling pathway in Helicobacter pylori-induced chronic atrophic gastritis. Authors - Tao Yang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

STAT6 Polyclonal antibody

STAT6 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis of NIH/3T3 using 51073-1-AP

WB analysis of NIH/3T3 using 51073-1-AP

WB analysis using 51073-1-AP

WB analysis using 51073-1-AP

WB analysis of MCF-7 using 51073-1-AP

IHC staining of human spleen using 51073-1-AP

IHC staining of human spleen using 51073-1-AP

IHC staining of human colon cancer using 51073-1-AP

IHC staining of mouse lung using 51073-1-AP

IHC staining of mouse lung using 51073-1-AP

IHC staining of mouse lung using 51073-1-AP

IHC staining of human lung using 51073-1-AP

IHC staining of human lung using 51073-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

miR-182 targeting reprograms tumor-associated macrophages and limits breast cancer progression.

Macrophage autophagy deficiency-induced CEBPB accumulation alleviates atopic dermatitis via impairing M2 polarization

Ehrlichia chaffeensis Tandem Repeat Effector Targets Differentially Influence Infection.

A miR-129-5P/ARID3A Negative Feedback Loop Modulates Diffuse Large B Cell Lymphoma Progression and Immune Evasion Through Regulating the PD-1/PD-L1 Checkpoint

Determination of WWOX Function in Modulating Cellular Pathways Activated by AP-2α and AP-2γ Transcription Factors in Bladder Cancer.

Berberine regulates macrophage polarization through IL-4-STAT6 signaling pathway in Helicobacter pylori-induced chronic atrophic gastritis.