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- KD/KO Validated
STAU1 Polyclonal antibody
STAU1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF/ICC, IP, RIP, ELISA
Conjugate
Unconjugated
Cat no : 14225-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human brain tissue, K-562 cells, mouse brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue, mouse pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 13 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
| RIP | See 4 publications below |
Product Information
14225-1-AP targets STAU1 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | STAU1 fusion protein Ag5464 相同性解析による交差性が予測される生物種 |
| Full Name | staufen, RNA binding protein, homolog 1 (Drosophila) |
| Calculated molecular weight | 63 kDa |
| Observed molecular weight | 55 kDa |
| GenBank accession number | BC050432 |
| Gene symbol | STAU1 |
| Gene ID (NCBI) | 6780 |
| RRID | AB_2302744 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Staufen1 (STAU1)-mediated mRNA decay (SMD) degrades translationally active mRNAs that bind the double-stranded (ds)RNA binding protein STAU1 within their 3'-untranslated regions (3'UTRs) [PMID:21307942]. STAU1 can binds to stem structures and protect Dvl2 mRNA from degradation by associating with the secondary structure in the U1 region which is located at 5′ position of second U stretch in the U2 region [PMID:17510634]. It has a role in specific positioning of mRNAs at given sites in the cell by cross-linking cytoskeletal and RNA components, and in stimulating their translation at the site. There are two isoforms of STAU1 protein and the molecular weight of two isoforms are repectively 63 kDa and 55 kDa
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for STAU1 antibody 14225-1-AP | Download protocol |
| IHC protocol for STAU1 antibody 14225-1-AP | Download protocol |
| IF protocol for STAU1 antibody 14225-1-AP | Download protocol |
| IP protocol for STAU1 antibody 14225-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nature hiCLIP reveals the in vivo atlas of mRNA secondary structures recognized by Staufen 1.
| ||
Cell Metab NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis. | ||
Mol Cell Gain of Additional BIRC3 Protein Functions through 3'-UTR-Mediated Protein Complex Formation. | ||
Nat Commun Genome-wide enhancer-gene regulatory maps link causal variants to target genes underlying human cancer risk | ||
Nucleic Acids Res Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR. |
