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STBD1 Polyclonal antibody

STBD1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 11842-1-AP

Synonyms

Genethonin 1, GENEX3414, GENX 3414, starch binding domain 1, STBD1


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Tested Applications

Positive WB detected inmouse skeletal muscle tissue, HeLa cells, A549 cells
Positive IP detected inA549 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11842-1-AP targets STBD1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STBD1 fusion protein Ag2481 相同性解析による交差性が予測される生物種
Full Name starch binding domain 1
Calculated molecular weight 41 kDa
Observed molecular weight 35-43 kDa
GenBank accession numberBC022301
Gene symbol STBD1
Gene ID (NCBI) 8987
RRIDAB_2197523
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

STBD1 may have the capability to bind to carbohydrates. It functions as a glycogen receptor that tethers glycogen to autophagic membranes for delivery and breakdown in lysosomes. STBD1 appears molecular mass of 35-38 kDa band in mouse/rat tissues and 38-43 kDa in human tissue.

Protocols

Product Specific Protocols
WB protocol for STBD1 antibody 11842-1-APDownload protocol
IHC protocol for STBD1 antibody 11842-1-APDownload protocol
IP protocol for STBD1 antibody 11842-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Cell

Glycogen accumulation and phase separation drives liver tumor initiation.

Authors - Qingxu Liu
human,mouseWB,IF

Redox Biol

Dysfunction of astrocytic glycophagy exacerbates reperfusion injury in ischemic stroke

Authors - Haiyun Guo
mouseWB

Acta Pharmacol Sin

Asiatic acid alleviates ischemic myocardial injury in mice by modulating mitophagy- and glycophagy-based energy metabolism.

Authors - Fan Qiu
mouseWB,IF

J Cell Sci

Stbd1 promotes glycogen clustering during endoplasmic reticulum stress and supports survival of mouse myoblasts

Authors - Andria A. Lytridou
  • KD Validated
mouseWB

Mol Endocrinol

Transgenic muscle specific Nor-1 expression regulates multiple pathways that effect adiposity, metabolism and endurance.

Authors - Pearen Michael A MA
WB,IF

J Cell Sci

Mouse Stbd1 is N-myristoylated and affects ER-mitochondria association and mitochondrial morphology.

Authors - Anthi Demetriadou