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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STBD1 Monoclonal antibody (67018-1-Ig)

    WB analysis using 67018-1-Ig (same clone as 67018-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67018-1-Ig (STBD1 antibody) at dilution of 1:16000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67018-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using STBD1 Monoclonal antibody (67018-1-Ig)

    IHC staining of human liver cancer using 67018-1-Ig (same clone as 67018-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67018-1-Ig (STBD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67018-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using STBD1 Monoclonal antibody (67018-1-Ig)

    IHC staining of human liver cancer using 67018-1-Ig (same clone as 67018-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67018-1-Ig (STBD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67018-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using STBD1 Monoclonal antibody (67018-1-Ig)

    IF Staining of HeLa using 67018-1-Ig (same clone as 67018-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using STBD1 antibody (67018-1-Ig, Clone: 1A2G2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67018-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50130-2

    Cytometric bead array standard curve of MP50130-2

    Cytometric bead array standard curve of MP50130-2, STBD1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67018-1-PBS. Detection antibody: 67018-2-PBS. Standard:Ag28613. Range: 1.563-100 ng/mL.

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STBD1 Monoclonal antibody, PBS Only (Capture)

STBD1 Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 67018-1-PBS
Clone No.1A2G2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

Starch-binding domain-containing protein 1, starch binding domain 1, Glycophagy cargo receptor STBD1, GENX-3414, Genethonin-1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67018-1-PBS targets STBD1 as part of a matched antibody pair:

MP50130-2: 67018-1-PBS capture and 67018-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen STBD1 fusion protein Ag28613 相同性解析による交差性が予測される生物種
Full Name starch binding domain 1
Calculated molecular weight 41 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC022301
Gene Symbol STBD1
Gene ID (NCBI) 8987
RRIDAB_2882334
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95210
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

STBD1 may have the capability to bind to carbohydrates. It functions as a glycogen receptor that tethers glycogen to autophagic membranes for delivery and breakdown in lysosomes. STBD1 appears molecular mass of 35-38 kDa band in mouse/rat tissues and 38-43 kDa in human tissue.