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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

STEAP2 Polyclonal antibody

STEAP2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24804-1-AP

Synonyms

PUMPCn, Protein up-regulated in metastatic prostate cancer, Prostate cancer-associated protein 1, PCANAP1, Metalloreductase STEAP2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STEAP2 Polyclonal antibody (24804-1-AP)

    WB analysis using 24804-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24804-1-AP (STEAP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using STEAP2 Polyclonal antibody (24804-1-AP)

    IHC staining of human colon cancer using 24804-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24804-1-AP (STEAP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using STEAP2 Polyclonal antibody (24804-1-AP)

    IHC staining of human colon cancer using 24804-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24804-1-AP (STEAP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inDU 145 cells, HEK-293T cells, U2OS cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:800-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24804-1-AP targets STEAP2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STEAP2 fusion protein Ag20293 相同性解析による交差性が予測される生物種
Full Name six transmembrane epithelial antigen of the prostate 2
Calculated molecular weight 490 aa, 56 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC148824
Gene symbol STEAP2
Gene ID (NCBI) 261729
RRIDAB_3085755
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

STEAP2, also named as PCANAP1 and STAMP1, belongs to the STEAP family. It is a metalloreductase that has the ability to reduce both Fe3+ to Fe2+ and Cu2+ to Cu1+. STEAP2 uses NAD+ as acceptor.

Protocols

Product Specific Protocols
WB protocol for STEAP2 antibody 24804-1-APDownload protocol
IHC protocol for STEAP2 antibody 24804-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols