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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using STEAP2-Specific Polyclonal antibody (20201-1-AP)

    WB analysis of HEK-293 using 20201-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20201-1-AP (STEAP2-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using STEAP2-Specific Polyclonal antibody (20201-1-AP)

    WB analysis of HeLa using 20201-1-AP

    WB result of STEAP2-Specific antibody (20201-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-STEAP2-Specific transfected HeLa cells.

  • Western Blot (WB) analysis of human brain tissue using STEAP2-Specific Polyclonal antibody (20201-1-AP)

    WB analysis of human brain using 20201-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 20201-1-AP (STEAP2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using STEAP2-Specific Polyclonal antibody (20201-1-AP)

    WB analysis of mouse kidney using 20201-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20201-1-AP (STEAP2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of DU 145 cells using STEAP2-Specific Polyclonal antibody (20201-1-AP)

    WB analysis of DU 145 using 20201-1-AP

    DU 145 cells were subjected to SDS PAGE followed by western blot with 20201-1-AP (STEAP2-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of fetal human brain tissue using STEAP2-Specific Polyclonal antibody (20201-1-AP)

    WB analysis of fetal human brain using 20201-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 20201-1-AP (STEAP2-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  • Featured Product
  • KD/KO Validated

STEAP2 Polyclonal antibody

STEAP2 Polyclonal Antibody for WB, ELISA
Cat No. 20201-1-AP
Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

STEAP2-Specific, PUMPCn, Protein up-regulated in metastatic prostate cancer, Prostate cancer-associated protein 1, PCANAP1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, DU 145 cells, fetal human brain tissue, mouse kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20201-1-AP targets STEAP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name six transmembrane epithelial antigen of the prostate 2
Calculated molecular weight 56 kDa
Observed molecular weight 48-52 kDa
GenBank accession numberNM_152999
Gene Symbol STEAP2
Gene ID (NCBI) 261729
RRIDAB_10665556
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NFT2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

STEAP2, also named as PCANAP1 and STAMP1, belongs to the STEAP family. It is a metalloreductase that has the ability to reduce both Fe3+ to Fe2+ and Cu2+ to Cu1+. STEAP2 uses NAD+ as acceptor. The antibody is specific to STEAP2.

Protocols

Product Specific Protocols
WB protocol for STEAP2 antibody 20201-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Exp Cell Res

Membrane cholesterol modulates STEAP2 conformation during dynamic intracellular trafficking processes leading to broad subcellular distribution.

Authors - Haruki Hasegawa
View Article
humanWB,IHC

Cell Death Dis

METTL3-mediated m6A modification of STEAP2 mRNA inhibits papillary thyroid cancer progress by blocking the Hedgehog signaling pathway and epithelial-to-mesenchymal transition.

Authors - Yue Zhu
  • KD Validated
View Article
humanWB

Cancer Biol Ther

STEAP2 promotes osteosarcoma progression by inducing epithelial–mesenchymal transition via the PI3K/AKT/mTOR signaling pathway and is regulated by EFEMP2

Authors - Dong Zhang
  • KD Validated
View Article
mouseWB

J Cell Mol Med

Unveiling the role of copper metabolism and STEAP2 in idiopathic pulmonary fibrosis molecular landscape

Authors - Yajun Wang
  • KD Validated
View Article