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KD/KO Validated

STEAP2 Polyclonal antibody

Name: STEAP2 Polyclonal antibody
Brand: Proteintech
SKU: 20201-1-AP

STEAP2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 20201-1-AP

Synonyms

STEAP2-Specific, PUMPCn, Protein up-regulated in metastatic prostate cancer, Prostate cancer-associated protein 1, PCANAP1

Validation Data Gallery

Filter:

AllWB

WB analysis of HEK-293 using 20201-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 20201-1-AP (STEAP2-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 20201-1-AP
WB result of STEAP2-Specific antibody (20201-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-STEAP2-Specific transfected HeLa cells.
WB analysis of DU 145 using 20201-1-AP
DU 145 cells were subjected to SDS PAGE followed by western blot with 20201-1-AP (STEAP2-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 20201-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 20201-1-AP (STEAP2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 20201-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20201-1-AP (STEAP2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of fetal human brain using 20201-1-AP
fetal human brain tissue were subjected to SDS PAGE followed by western blot with 20201-1-AP (STEAP2-Specific Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Cat No. 20201-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in

HEK-293 cells, HeLa cells, DU 145 cells, fetal human brain tissue, mouse kidney tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 4 publications below
IHC	See 1 publications below

Product Information

20201-1-AP targets STEAP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	six transmembrane epithelial antigen of the prostate 2
Calculated molecular weight	56 kDa
Observed molecular weight	56 kDa
GenBank accession number	NM_152999
Gene symbol	STEAP2
Gene ID (NCBI)	261729
RRID	AB_10665556
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

STEAP2, also named as PCANAP1 and STAMP1, belongs to the STEAP family. It is a metalloreductase that has the ability to reduce both Fe3+ to Fe2+ and Cu2+ to Cu1+. STEAP2 uses NAD+ as acceptor. The antibody is specific to STEAP2.

Protocols

Product Specific Protocols
WB protocol for STEAP2 antibody 20201-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Exp Cell Res Membrane cholesterol modulates STEAP2 conformation during dynamic intracellular trafficking processes leading to broad subcellular distribution. Authors - Haruki Hasegawa View Article
human	WB,IHC	Cell Death Dis METTL3-mediated m6A modification of STEAP2 mRNA inhibits papillary thyroid cancer progress by blocking the Hedgehog signaling pathway and epithelial-to-mesenchymal transition. Authors - Yue Zhu KD Validated View Article
human	WB	Cancer Biol Ther STEAP2 promotes osteosarcoma progression by inducing epithelial–mesenchymal transition via the PI3K/AKT/mTOR signaling pathway and is regulated by EFEMP2 Authors - Dong Zhang KD Validated View Article
mouse	WB	J Cell Mol Med Unveiling the role of copper metabolism and STEAP2 in idiopathic pulmonary fibrosis molecular landscape Authors - Yajun Wang KD Validated View Article

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研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

STEAP2 Polyclonal antibody

STEAP2 Polyclonal Antibody for WB, ELISA

Validation Data Gallery

WB analysis of HEK-293 using 20201-1-AP

WB analysis of HeLa using 20201-1-AP

WB analysis of DU 145 using 20201-1-AP

WB analysis of human brain using 20201-1-AP

WB analysis of mouse kidney using 20201-1-AP

WB analysis of fetal human brain using 20201-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Membrane cholesterol modulates STEAP2 conformation during dynamic intracellular trafficking processes leading to broad subcellular distribution.

METTL3-mediated m6A modification of STEAP2 mRNA inhibits papillary thyroid cancer progress by blocking the Hedgehog signaling pathway and epithelial-to-mesenchymal transition.

STEAP2 promotes osteosarcoma progression by inducing epithelial–mesenchymal transition via the PI3K/AKT/mTOR signaling pathway and is regulated by EFEMP2

Unveiling the role of copper metabolism and STEAP2 in idiopathic pulmonary fibrosis molecular landscape