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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STEAP3 Recombinant antibody (83484-2-RR)

    WB analysis using 83484-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83484-2-RR (STEAP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using STEAP3 Recombinant antibody (83484-2-RR)

    FC experiment of HeLa using 83484-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug STEAP3 Recombinant antibody (83484-2-RR, Clone:1N5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83484-2-RR

    Affinity and Kinetic Characterization of 83484-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83484-2-RR against Human STEAP3 were performed. The affinity constant is 0.89 nM.

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STEAP3 Recombinant antibody

STEAP3 Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83484-2-RR
Clone No.1N5

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, FC (Intra), ELISA

Synonyms

hpHyde, EC:1.16.1.-, Dudulin-2, Dudulin 2, dudlin 2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, PC-12 cells, C6 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83484-2-RR targets STEAP3 in WB, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen STEAP3 fusion protein Ag29528 相同性解析による交差性が予測される生物種
Full Name STEAP family member 3
Calculated molecular weight 488 aa, 55 kDa
Observed molecular weight55 kDa
GenBank accession numberBC042150
Gene Symbol STEAP3
Gene ID (NCBI) 55240
RRIDAB_3671112
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ658P3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

STEAP3 (Six-Transmembrane Epithelial Antigen of Prostate 3) is also named as TSAP6, Dudulin-2 and pHyde, and belongs to the STEAP family. STEAP3 is a member of the STEAP family and is composed of a six-transmembrane domain at the COOH-terminal domain and a cytoplasmic N-terminal oxidoreductase domain, which is essential for iron and copper uptake (PMID:16227996). STEAP3 contains a functional p53-binding site in its promoter and can be upregulated following p53 activation to enhance cell death in myeloid leukemia cell line and breast cancer cells (PMID: 18617898). By interacting with Nix, a pro-apoptotic Bcl-2 family member, and Myt1 kinase, a negative regulator of the G2/M transition, STEAP3 overexpression promotes apoptosis and inhibits G2/M transition in cell cycle progression (PMID: 12606722, PMID: 10504341).

Protocols

Product Specific Protocols
WB protocol for STEAP3 antibody 83484-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols