- Featured Product
- KD/KO Validated
STIM2 Polyclonal antibody
STIM2 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
Cat no : 21192-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, Jurkat cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | mouse spleen tissue, human brain tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 10 publications below |
| IF | See 3 publications below |
| IP | See 1 publications below |
Product Information
The immunogen of 21192-1-AP is STIM2 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | STIM2 fusion protein Ag15530 相同性解析による交差性が予測される生物種 |
| Full Name | stromal interaction molecule 2 |
| Calculated molecular weight | 754 aa, 85 kDa |
| Observed molecular weight | 85-115 kDa |
| GenBank accession number | BC146661 |
| Gene symbol | STIM2 |
| Gene ID (NCBI) | 57620 |
| RRID | AB_10734322 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
STIM2, also named as KIAA1482, functions as a highly sensitive Ca2+ sensor in the endoplasmic reticulum which activates both store-operated and store-independent Ca2+-influx. Regulates basal cytosolic and endoplasmic reticulum Ca2+ concentrations. Upon mild variations of the endoplasmic reticulum Ca2+ concentration, STIM2 translocates from the endoplasmic reticulum to the plasma membrane where it probably activates the Ca2+ release-activated Ca2+ (CRAC) channels ORAI1, ORAI2 and ORAI3. STIM2 may inhibit STIM1-mediated Ca2+ influx. STIM2 has 2 isoforms 105kd and 115kd with Differential levels of phosphorylation.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for STIM2 antibody 21192-1-AP | Download protocol |
| IHC protocol for STIM2 antibody 21192-1-AP | Download protocol |
| IP protocol for STIM2 antibody 21192-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Res Cholesterylation of Smoothened is a calcium-accelerated autoreaction involving an intramolecular ester intermediate.
| ||
Cell Mol Life Sci STIM2 regulates NMDA receptor endocytosis that is induced by short-term NMDA receptor overactivation in cortical neurons
| ||
Oncotarget The calcium channel proteins ORAI3 and STIM1 mediate TGF-β induced Snai1 expression.
| ||
Neurobiol Dis Inhibiting store-operated calcium entry attenuates white matter secondary degeneration following SCI. | ||
Biochem J STIM1 negatively regulates Ca2+ release from the sarcoplasmic reticulum in skeletal myotubes. | ||
Cell Signal RGS10 physically and functionally interacts with STIM2 and requires store-operated calcium entry to regulate proinflammatory gene expression in microglia.
|
