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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STIP1 Monoclonal antibody (68155-1-Ig)

    WB analysis using 68155-1-Ig (same clone as 68155-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68155-1-Ig (STIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68155-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using STIP1 Monoclonal antibody (68155-1-Ig)

    IP experiment of HeLa using 68155-1-Ig (same clone as 68155-1-PBS)

    IP result of anti-STIP1 (IP:68155-1-Ig, 5ug; Detection:68155-1-Ig 1:1000) with HeLa cells lysate 1880 ug. This data was developed using the same antibody clone with 68155-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Jurkat cells using STIP1 Monoclonal antibody (68155-1-Ig)

    IP experiment of Jurkat using 68155-1-Ig (same clone as 68155-1-PBS)

    IP result of anti-STIP1 (IP:68155-1-Ig, 5ug; Detection:68155-1-Ig 1:1000) with Jurkat cells lysate 2400 ug. This data was developed using the same antibody clone with 68155-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using STIP1 Monoclonal antibody (68155-1-Ig)

    IHC staining of mouse liver using 68155-1-Ig (same clone as 68155-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68155-1-Ig (STIP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68155-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using STIP1 Monoclonal antibody (68155-1-Ig)

    IHC staining of mouse liver using 68155-1-Ig (same clone as 68155-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68155-1-Ig (STIP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68155-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using STIP1 Monoclonal antibody (68155-1-Ig)

    IF Staining of HeLa using 68155-1-Ig (same clone as 68155-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using STIP1 antibody (68155-1-Ig, Clone: 1B5H2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68155-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using STIP1 Monoclonal antibody (68155-1-Ig)

    FC experiment of MCF-7 using 68155-1-Ig (same clone as 68155-1-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.4 ug STIP1 Monoclonal antibody (68155-1-Ig, Clone:1B5H2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68155-1-PBS in a different storage buffer formulation.

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STIP1 Monoclonal antibody, PBS Only

STIP1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 68155-1-PBS
Clone No.1B5H2

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

Renal carcinoma antigen NY-REN-11, P60, IEF SSP 3521, Hsc70/Hsp90-organizing protein, Hsc70/Hsp90 organizing protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68155-1-PBS targets STIP1 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen STIP1 fusion protein Ag7851 相同性解析による交差性が予測される生物種
Full Name stress-induced-phosphoprotein 1
Calculated molecular weight 63 kDa
Observed molecular weight61 kDa
GenBank accession numberBC002987
Gene Symbol STIP1
Gene ID (NCBI) 10963
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP31948
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

STIP1, also named as Hop and NY-REN-11. Its expression is low in the hypothalamus. STIP1 has some isoforms with MW 62 kDa, 68 kDa and 59 kDa. Acts as a co-chaperone for HSP90AA1.Mediates the association of the molecular chaperones HSPA8/HSC70 and HSP90, Probably forms a complex composed of chaperones HSP90 and HSP70, co-chaperones STIP1/HOP, CDC37, PPP5C, PTGES3/p23, TSC1 and client protein TSC2. STIP1 interacts with PACRG, EEF1AKMT3, FLCN, FNIP1 and FNIP2 (PMID:24880080).