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Validation Data Gallery

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  • Western Blot (WB) analysis of SKOV-3 cells using STK10 Polyclonal antibody (25471-1-AP)

    WB analysis of SKOV-3 using 25471-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 25471-1-AP (STK10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using STK10 Polyclonal antibody (25471-1-AP)

    WB analysis of A549 using 25471-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 25471-1-AP (STK10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using STK10 Polyclonal antibody (25471-1-AP)

    WB analysis of A549 using 25471-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 25471-1-AP (STK10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using STK10 Polyclonal antibody (25471-1-AP)

    WB analysis of COLO 320 using 25471-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 25471-1-AP (STK10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using STK10 Polyclonal antibody (25471-1-AP)

    IHC staining of human lymphoma using 25471-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25471-1-AP (STK10 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using STK10 Polyclonal antibody (25471-1-AP)

    IHC staining of human lymphoma using 25471-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25471-1-AP (STK10 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  • Featured Product
  • KD/KO Validated

STK10 Polyclonal antibody

STK10 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25471-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

serine/threonine kinase 10, PRO2729, Lymphocyte-oriented kinase, Lymphocyte oriented kinase, LOK

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inSKOV-3 cells, A549 cells, COLO 320 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25471-1-AP targets STK10 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STK10 fusion protein Ag22169 相同性解析による交差性が予測される生物種
Full Name serine/threonine kinase 10
Calculated molecular weight 968 aa, 112 kDa
Observed molecular weight 130 kDa
GenBank accession numberBC070077
Gene Symbol STK10
Gene ID (NCBI) 6793
RRIDAB_2880096
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO94804
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Lymphocyte-oriented kinase deficiency encoded by the serine/threonine kinase 10 (STK10) gene correlates with the intracellular adhesion molecule 1 (ICAM-1)/lymphocyte function associated antigen 1 (LFA-1) complex in aspirin hypersensitivity(PMID:21905501). STK 10 is also named as LOK and expressed as a 130-kDa protein, which was detected predominantly in lymphoid organs such as spleen, thymus, and bone marrow, in contrast to other mammalian members of the STE20 family(PMID:9278426).

Protocols

Product Specific Protocols
WB protocol for STK10 antibody 25471-1-APDownload protocol
IHC protocol for STK10 antibody 25471-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Immunol

The enhancement of immunoactivity induced by immunogenic cell death through serine/threonine kinase 10 inhibition: a potential therapeutic strategy

Authors - Xiaoli Xia
  • KD Validated
View Article

Clin Cancer Res

Combined CDK4/6 and ERK1/2 inhibition enhances anti-tumor activity in NF1-associated plexiform neurofibroma

Authors - Alyssa C Flint
View Article