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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

STK11/LKB1 Monoclonal antibody, PBS Only

STK11/LKB1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D11E5

Cat no : 68016-1-PBS

Synonyms

LKB1, STK11, STK11/ LKB1, PJS, Liver kinase B1

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using STK11/LKB1 Monoclonal antibody (68016-1-Ig)

    WB analysis of HEK-293 using 68016-1-Ig (same clone as 68016-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 68016-1-Ig (STK11/LKB1 antibody) at dilution of 1:4900 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68016-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using STK11/LKB1 Monoclonal antibody (68016-1-Ig)

    WB analysis of NIH/3T3 using 68016-1-Ig (same clone as 68016-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68016-1-Ig (STK11/LKB1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68016-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using STK11/LKB1 Monoclonal antibody (68016-1-Ig)

    WB analysis of PC-3 using 68016-1-Ig (same clone as 68016-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 68016-1-Ig (STK11/LKB1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68016-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using STK11/LKB1 Monoclonal antibody (68016-1-Ig)

    WB analysis of HSC-T6 using 68016-1-Ig (same clone as 68016-1-PBS)

    Calyculin A treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68016-1-Ig (STK11/LKB1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68016-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using STK11/LKB1 Monoclonal antibody (68016-1-Ig)

    IHC staining of mouse testis using 68016-1-Ig (same clone as 68016-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68016-1-Ig (STK11/LKB1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68016-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using STK11/LKB1 Monoclonal antibody (68016-1-Ig)

    IHC staining of mouse testis using 68016-1-Ig (same clone as 68016-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68016-1-Ig (STK11/LKB1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68016-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse colon tissue using STK11/LKB1 Monoclonal antibody (68016-1-Ig)

    IF Staining of mouse colon using 68016-1-Ig (same clone as 68016-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using STK11/LKB1 antibody (68016-1-Ig, Clone: 2D11E5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68016-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SKOV-3 cells using STK11/LKB1 Monoclonal antibody (68016-1-Ig)

    IF Staining of SKOV-3 using 68016-1-Ig (same clone as 68016-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using STK11/LKB1 antibody (68016-1-Ig, Clone: 2D11E5 ) at dilution of 1:500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 68016-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68016-1-PBS targets STK11/LKB1 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name serine/threonine kinase 11
Calculated molecular weight 49 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC007981
Gene symbol LKB1
Gene ID (NCBI) 6794
RRIDAB_2918762
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

STK11(serine/threonine-protein kinase 11) is also named as LKB1, PJS, and belongs to the protein kinase superfamily. It controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. The tumour suppressor protein LKB1 is a serine/threonine kinase that has been causally linked to Peutz-Jeghers syndrome (PJS). Defects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) and defects in STK11 have been associated with testicular germ cell tumor (TGCT) and some sporadic cancers, especially lung cancers. STK11 has 2 isoforms with MW of 49 kDa and 45 kDa, and can be detected as 50-54 kDa after posttranslational modification.