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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

STK33 Polyclonal antibody

STK33 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 12857-1-AP

Synonyms

Serine/threonine-protein kinase 33, serine/threonine kinase 33, EC:2.7.11.1

Validation Data Gallery

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  • Western Blot (WB) analysis of human kidney tissue using STK33 Polyclonal antibody (12857-1-AP)

    WB analysis of human kidney using 12857-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 12857-1-AP (STK33 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using STK33 Polyclonal antibody (12857-1-AP)

    WB analysis of mouse testis using 12857-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12857-1-AP (STK33 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using STK33 Polyclonal antibody (12857-1-AP)

    IP experiment of HEK-293 using 12857-1-AP

    IP result of anti-STK33 (IP:12857-1-AP, 4ug; Detection:12857-1-AP 1:300) with HEK-293 cells lysate 880ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using STK33 Polyclonal antibody (12857-1-AP)

    IHC staining of human liver cancer using 12857-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12857-1-AP (STK33 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using STK33 Polyclonal antibody (12857-1-AP)

    IHC staining of human liver cancer using 12857-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12857-1-AP (STK33 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using STK33 Polyclonal antibody (12857-1-AP)

    IF Staining of A431 using 12857-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using STK33 antibody (12857-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

Positive WB detected inhuman kidney tissue, mouse testis tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12857-1-AP targets STK33 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STK33 fusion protein Ag3561 相同性解析による交差性が予測される生物種
Full Name serine/threonine kinase 33
Calculated molecular weight 514 aa, 58 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC031231
Gene symbol STK33
Gene ID (NCBI) 65975
RRIDAB_10642563
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Serine/threonine kinase 33 (STK33) gene, which is identified in human chromosome 11 and mouse chromosome 7, encodes a serine/threonine kinase. STK33 belongs to a member of the calcium/calmodulin‐dependent kinase (CAMK), which is mainly existed in testis, certain brain regions and embryonic organs such as brain, heart and spinal cord in human and mouse and is likely to participate in spermatogenesis and organ ontogenesis. (PMID: 30256516, PMID: 34536775)

Protocols

Product Specific Protocols
WB protocol for STK33 antibody 12857-1-APDownload protocol
IHC protocol for STK33 antibody 12857-1-APDownload protocol
IF protocol for STK33 antibody 12857-1-APDownload protocol
IP protocol for STK33 antibody 12857-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Cell Proteomics

STK33 phosphorylates fibrous sheath protein AKAP3/4 to regulate sperm flagella assembly in spermiogenesis

Authors - Weiling Yu
View Article