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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

STK38 Polyclonal antibody

STK38 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 55335-1-AP

Synonyms

Nuclear Dbf2-related kinase 1, Nuclear Dbf2 related kinase 1, NDR1 protein kinase, NDR1, NDR

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using STK38 Polyclonal antibody (55335-1-AP)

    WB analysis of MCF-7 using 55335-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 55335-1-AP (STK38 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using STK38 Polyclonal antibody (55335-1-AP)

    WB analysis of MCF-7 using 55335-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 55335-1-AP (STK38 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using STK38 Polyclonal antibody (55335-1-AP)

    WB analysis of HeLa using 55335-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 55335-1-AP (STK38 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using STK38 Polyclonal antibody (55335-1-AP)

    IHC staining of human kidney using 55335-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55335-1-AP (STK38 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using STK38 Polyclonal antibody (55335-1-AP)

    IHC staining of human kidney using 55335-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55335-1-AP (STK38 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using STK38 Polyclonal antibody (55335-1-AP)

    IHC staining of human breast cancer using 55335-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55335-1-AP (STK38 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using STK38 Polyclonal antibody (55335-1-AP)

    IHC staining of human breast cancer using 55335-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55335-1-AP (STK38 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using STK38 Polyclonal antibody (55335-1-AP)

    IF Staining of HEK-293 using 55335-1-AP

    Immunofluorescent analysis of HEK-293 cells using 55335-1-AP (STK38 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells
Positive IHC detected inhuman kidney tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55335-1-AP targets STK38 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name serine/threonine kinase 38
Calculated molecular weight 54 kDa
Observed molecular weight 54 kDa
GenBank accession numberNM_007271
Gene symbol STK38
Gene ID (NCBI) 11329
RRIDAB_2881310
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Serine/threonine-protein kinase 38 belongs to AGC Ser/Thr protein kinase family.STK38 implicates in cell proliferation and/or tumor progression, and might play a role in the HIV-1 life cycle.Researches showed that STK38 with STK38L, were proapoptotic kinases and key members of the RASSF1/STK4 signaling cascade and its kinase activity increased in early mitotic phase and was dependent on FRY and STK3.This antibody recognizes the C-terminal of STK38.

Protocols

Product Specific Protocols
WB protocol for STK38 antibody 55335-1-APDownload protocol
IHC protocol for STK38 antibody 55335-1-APDownload protocol
IF protocol for STK38 antibody 55335-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF, WB, IHC

J Cell Biochem

Phosphorylated LASS2 inhibits prostate carcinogenesis via negative regulation of Wnt/β-catenin signaling.

Authors - Kuangen Zhang
View Article
humanWB,IF

J Proteome Res

ZSWIM1 Promotes the Proliferation and Metastasis of Lung Adenocarcinoma Cells through the STK38/MEKK2/ERK1/2 Axis

Authors - Xuejuan Gao
View Article
humanWB,IP

Nat Commun

A bacterial autotransporter impairs innate immune responses by targeting the transcription factor TFE3

Authors - Atri Ta
View Article