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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STK39 Recombinant antibody (83081-2-RR)

    WB analysis using 83081-2-RR (same clone as 83081-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83081-2-RR (STK39 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83081-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00106-3

    Sandwich ELISA standard curve of MP00106-3

    Sandwich ELISA standard curve of MP00106-3, Human STK39 Recombinant Matched Antibody Pair - PBS only. 83081-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag34503. 83081-1-PBS was HRP conjugated as the detection antibody. Range: 31.3-2000 pg/mL.

  • Sample test of MP00106-3

    Sample test of MP00106-3

    The mean STK39 concentration was determined to be 2,623.5 pg/mL in HepG2 cell extract based on a 4.2 mg/mL extract load and 9,245.2 pg/mL in Jurkat cell extract based on a 4.6 mg/mL extract load.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using STK39 Recombinant antibody (83081-2-RR)

    IF Staining of mouse brain using 83081-2-RR (same clone as 83081-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using STK39 antibody (83081-2-RR, Clone: 230382G9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83081-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using STK39 Recombinant antibody (83081-2-RR)

    IF Staining of NIH/3T3 using 83081-2-RR (same clone as 83081-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using STK39 antibody (83081-2-RR, Clone: 230382G9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83081-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00106-2

    Cytometric bead array standard curve of MP00106-2

    Cytometric bead array standard curve of MP00106-2, STK39 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83081-2-PBS. Detection antibody: 83081-4-PBS. Standard: Ag34503. Range: 0.625-20 ng/mL.

  • Affinity and Kinetic Characterization of 83081-2-RR

    Affinity and Kinetic Characterization of 83081-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83081-2-RR against Human STK39 were performed. The affinity constant is 2.52 nM.

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STK39 Recombinant antibody, PBS Only (Capture)

STK39 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83081-2-PBS
Clone No.230382G9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IF-P, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Ste 20 related kinase, SPAK, PASK, DCHT, 230382G9

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83081-2-PBS targets STK39 as part of a matched antibody pair:

MP00106-2: 83081-2-PBS capture and 83081-4-PBS detection (validated in Cytometric bead array)

MP00106-3: 83081-2-PBS capture and 83081-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen STK39 fusion protein Ag34503 相同性解析による交差性が予測される生物種
Full Name serine threonine kinase 39 (STE20/SPS1 homolog, yeast)
Calculated molecular weight59 kDa
Observed molecular weight62 kDa
GenBank accession numberBC166614
Gene Symbol STK39
Gene ID (NCBI) 27347
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UEW8
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.

Background Information

STK39 (Serine/Threonine Kinase 39) is a proline- and alanine-rich Ste20-related kinase (also named as SPAK), which is composed of a short N-terminal proline and alanine repeats (PAPA box), a kinase catalytic domain and a C-terminal regulatory domain (PMID: 16382158). In mammalians, STK39 plays an important role in ion homeostasis by regulating the cation chloride cotransporters' activities, which is crucial for the modulation of renal salt transport and blood pressure (PMID: 33500714). Notably, STK39 is also implicated in the regulation of tumor cell proliferation, migration, and invasion in multiple cancers, including osteosarcoma and cervical cancer (PMID: 34335956).