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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using Stathmin 1 Monoclonal antibody (66090-1-Ig)

    WB analysis of Jurkat using 66090-1-Ig

    WB result of Stathmin antibody (66090-1-Ig, 1:4000) with si-Control and si-Stathmin transfected Jurkat cells.

  • Western Blot (WB) analysis of Jurkat cells using Stathmin 1 Monoclonal antibody (66090-1-Ig)

    WB analysis of Jurkat using 66090-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66090-1-Ig (Stathmin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using Stathmin 1 Monoclonal antibody (66090-1-Ig)

    WB analysis of human brain using 66090-1-Ig

    human brain tissue were subjected to SDS PAGE followed by western blot with 66090-1-Ig (Stathmin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using Stathmin 1 Monoclonal antibody (66090-1-Ig)

    WB analysis of K-562 using 66090-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 66090-1-Ig (Stathmin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using Stathmin 1 Monoclonal antibody (66090-1-Ig)

    IHC staining of human testis using 66090-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis slide using 66090-1-Ig (Stathmin antibody at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of mouse olfactory epithelium tissue using Stathmin 1 Monoclonal antibody (66090-1-Ig)

    IF Staining of mouse olfactory epithelium tissue using 66090-1-Ig

    Fluorescent IHC on 1%PLP fixed frozen mouse olfactory epithelium tissue of STMN1 antibody (66090-1-IG, 1:200). Basal cell staining and dendritic processes as expected. By Dr. Brian Lin (Schwob Lab).

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  • Featured Product
  • KD/KO Validated

Stathmin 1 Monoclonal antibody

Stathmin 1 Monoclonal Antibody for WB, IF, IHC, ELISA
Cat No. 66090-1-Ig
Clone No.7D1E1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, K-562 cells, human brain tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66090-1-Ig targets Stathmin 1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Stathmin 1 fusion protein Ag19102 相同性解析による交差性が予測される生物種
Full Name stathmin 1/oncoprotein 18
Calculated molecular weight 18 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC014353
Gene Symbol Stathmin 1
Gene ID (NCBI) 3925
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP16949
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Stathmin 1 (STMN1) normally regulates microtubule dynamics either by sequestering free tubulin heterodimers or by promoting microtubule catastrophe. STMN1 is highly expressed in fetal and adult brain, spinal cord, and cerebellum. Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. Phosphorylation of stathmin is involved in response to NGF, neuron polarization and microtubule polymerization inhibition activity. Increased expression of STMN1 has been observed in a variety of human malignancies, such as colorectal primary tumors and metastatic tissues, but its association with melanoma is so far not well known.