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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using STMN2 Recombinant antibody (82925-3-RR)

    IF Staining of SH-SY5Y using 82925-3-RR (same clone as 82925-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using STMN2 antibody (82925-3-RR, Clone: 230110G10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82925-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using STMN2 Recombinant antibody (82925-3-RR)

    FC experiment of SH-SY5Y using 82925-3-RR (same clone as 82925-3-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug STMN2 Recombinant antibody (82925-3-RR, Clone:230110G10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82925-3-PBS in a different storage buffer formulation.

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STMN2 Recombinant antibody, PBS Only

STMN2 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82925-3-PBS
Clone No.230110G10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Synonyms

SGC 10, SCGN10, SCGN 10, SCG10, SCG 10

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82925-3-PBS targets STMN2 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen STMN2 fusion protein Ag0929 相同性解析による交差性が予測される生物種
Full Name stathmin-like 2
Calculated molecular weight 21 kDa
GenBank accession numberBC006302
Gene Symbol STMN2
Gene ID (NCBI) 11075
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ93045
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Stathmin-2 (encoded by STMN2), a member of the stathmin family of proteins, encodes a phosphoprotein that has an important role in microtubule dynamics. The STMN2 protein promotes microtubule dynamics in axonal growth cones , and is thought to play an important role in neurite outgrowth. STMN2 protein is localized in both Golgi and cytosolic compartments. Its expression appears modulated in osteoblasts by nerve growth factor, dexamethasone or RhoA kinase inhibitor Y-27632 which are known effectors of osteogenesis. (PMID: 33841129, PMID: 18611392)