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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

STOML1 Polyclonal antibody

STOML1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12862-1-AP

Synonyms

EPB72 like protein 1, hUNC 24, Protein unc 24 homolog, SLP 1, SLP1, stomatin (EPB72) like 1, Stomatin like protein 1, Stomatin related protein, STOML1, STORP, UNC24

Validation Data Gallery

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  • Western Blot (WB) analysis of A375 cells using STOML1 Polyclonal antibody (12862-1-AP)

    WB analysis of A375 using 12862-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 12862-1-AP (STOML1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using STOML1 Polyclonal antibody (12862-1-AP)

    WB analysis of human brain using 12862-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12862-1-AP (STOML1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using STOML1 Polyclonal antibody (12862-1-AP)

    IHC staining of human gliomas using 12862-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12862-1-AP (STOML1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using STOML1 Polyclonal antibody (12862-1-AP)

    IHC staining of human gliomas using 12862-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12862-1-AP (STOML1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inA375 cells, human brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12862-1-AP targets STOML1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STOML1 fusion protein Ag3978 相同性解析による交差性が予測される生物種
Full Name stomatin (EPB72)-like 1
Calculated molecular weight 45 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC034379
Gene symbol STOML1
Gene ID (NCBI) 9399
RRIDAB_2197415
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for STOML1 antibody 12862-1-APDownload protocol
IHC protocol for STOML1 antibody 12862-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols