Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, C6 cells, HEK-293 cells, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human stomach cancer tissue, human endometrial cancer tissue, human breast cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 7 publications below |
| WB | See 40 publications below |
| IHC | See 24 publications below |
| IF | See 6 publications below |
| IP | See 2 publications below |
Product Information
10348-1-AP targets STOML2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | STOML2 fusion protein Ag0363 相同性解析による交差性が予測される生物種 |
| Full Name | stomatin (EPB72)-like 2 |
| Calculated molecular weight | 356 aa, 39 kDa |
| Observed molecular weight | 39 kDa |
| GenBank accession number | BC002442 |
| Gene Symbol | STOML2 |
| Gene ID (NCBI) | 30968 |
| RRID | AB_2286822 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UJZ1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
STOML2 (Stomatin-like protein 2; also known as SLP-2) is a widely expressed mitochondrial member of the highly conserved family of stomatin proteins. STOML2 is localized mostly in mitochondrial inner membrane, minor in plasma membrane. Human STOML2 interacts with prohibitins and regulates mitochondrial biogenesis and function (21746876). In addition, plenty of studies revealed that STOML2 was overexpressed in many human cancer tissues, and its expression may be a new valuable prognostic biomarker (19839737, 21960069). Recently STOML2 has been identified as a serological biomarker for early colorectal cancer (CRC) diagnosis (21209152). Two isoforms of STOML2 exist due to the alternative splicing, with MW of 39 kDa and 33 kDa, respectively.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for STOML2 antibody 10348-1-AP | Download protocol |
| IHC protocol for STOML2 antibody 10348-1-AP | Download protocol |
| IF protocol for STOML2 antibody 10348-1-AP | Download protocol |
| IP protocol for STOML2 antibody 10348-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria | ||
Cell Metab DNAJC19, a mitochondrial cochaperone associated with cardiomyopathy, forms a complex with prohibitins to regulate cardiolipin remodeling. | ||
Clin Cancer Res Stomatin-like protein 2 is overexpressed in cancer and involved in regulating cell growth and cell adhesion in human esophageal squamous cell carcinoma. | ||
EMBO Rep The membrane scaffold SLP2 anchors a proteolytic hub in mitochondria containing PARL and the i-AAA protease YME1L. | ||
Hum Mol Genet Disease mutations in Rab7 result in unregulated nucleotide exchange and inappropriate activation. | ||
J Pathol Knockdown of stomatin-like protein 2 (STOML2) reduces the invasive ability of glioma cells through inhibition of the NF-κB/MMP-9 pathway. |