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Validation Data Gallery

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  • Western Blot (WB) analysis of pig brain tissue using STOML2 Monoclonal antibody (60052-1-Ig)

    WB analysis of pig brain using 60052-1-Ig (same clone as 60052-1-PBS)

    Pig, rat, and mouse brain tissues were subjected to SDS PAGE followed by western blot with 60052-1-Ig (STOML2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60052-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using STOML2 Monoclonal antibody (60052-1-Ig)

    WB analysis of MCF-7 using 60052-1-Ig (same clone as 60052-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 60052-1-Ig (STOML2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60052-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using STOML2 Monoclonal antibody (60052-1-Ig)

    WB analysis of HepG2 using 60052-1-Ig (same clone as 60052-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 60052-1-Ig (STOML2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60052-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of RAW 264.7 cells using STOML2 Monoclonal antibody (60052-1-Ig)

    WB analysis of RAW 264.7 using 60052-1-Ig (same clone as 60052-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60052-1-Ig (STOML2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60052-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using STOML2 Monoclonal antibody (60052-1-Ig)

    IP experiment of mouse brain using 60052-1-Ig (same clone as 60052-1-PBS)

    IP result of anti-STOML2 (IP:60052-1-Ig, 4ug; Detection:60052-1-Ig 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 60052-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using STOML2 Monoclonal antibody (60052-1-Ig)

    IHC staining of human stomach cancer using 60052-1-Ig (same clone as 60052-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60052-1-Ig (STOML2 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60052-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using STOML2 Monoclonal antibody (60052-1-Ig)

    IHC staining of human stomach cancer using 60052-1-Ig (same clone as 60052-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60052-1-Ig (STOML2 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60052-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using STOML2 Monoclonal antibody (60052-1-Ig)

    IHC staining of human endometrial cancer using 60052-1-Ig (same clone as 60052-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 60052-1-Ig (STOML2 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60052-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using STOML2 Monoclonal antibody (60052-1-Ig)

    IHC staining of human endometrial cancer using 60052-1-Ig (same clone as 60052-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 60052-1-Ig (STOML2 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60052-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using STOML2 Monoclonal antibody (60052-1-Ig)

    IF Staining of A431 using 60052-1-Ig (same clone as 60052-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using STOML2 antibody (60052-1-Ig, Clone: 1A2E9 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 60052-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

STOML2 Monoclonal antibody, PBS Only

STOML2 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 60052-1-PBS
Clone No.1A2E9

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

SLP 2, Paratarg-7, Paraprotein target 7, EPB72-like protein 2, EPB72 like protein 2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60052-1-PBS targets STOML2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen STOML2 fusion protein Ag0363 相同性解析による交差性が予測される生物種
Full Name stomatin (EPB72)-like 2
Calculated molecular weight 356 aa, 39 kDa
Observed molecular weight 39 kDa
GenBank accession numberBC002442
Gene Symbol STOML2
Gene ID (NCBI) 30968
RRIDAB_2271235
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UJZ1
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Human stomatin (band 7.2b) is a 31-kDa erythrocyte membrane protein of unknown function but implicated in the control of ion channel permeability, mechanoreception, and lipid domain organization. Stomatin (EPB72)-like 2 (STOML2, synonyms: SLP-2, HSPC108) is a 38.5-kDa protein that is overall approximately 20% similar to human stomatin. STOML2 is also present in mature human erythrocytes , but lacks a characteristic NH(2)-terminal hydrophobic domain found in other stomatin homologues. STOML2 may link stomatin or other integral membrane proteins to the peripheral cytoskeleton and thereby play a role in regulating ion channel conductances or the organization of sphingolipid and cholesterol-rich lipid rafts.