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  • Featured Product
  • KD/KO Validated

STRAP Monoclonal antibody, PBS Only

STRAP Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5E11F3

Cat no : 66712-1-PBS

Synonyms

MAP activator with WD repeats, MAWD, PT WD, STRAP, UNR interacting protein, UNRIP, WD 40 repeat protein PT WD

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STRAP Monoclonal antibody (66712-1-Ig)

    WB analysis using 66712-1-Ig (same clone as 66712-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66712-1-Ig (STRAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66712-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using STRAP Monoclonal antibody (66712-1-Ig)

    WB analysis of HEK-293 using 66712-1-Ig (same clone as 66712-1-PBS)

    WB result of STRAP antibody (66712-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STRAP transfected HEK-293 cells. This data was developed using the same antibody clone with 66712-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using STRAP Monoclonal antibody (66712-1-Ig)

    IHC staining of mouse ovary using 66712-1-Ig (same clone as 66712-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 66712-1-Ig (STRAP antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66712-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using STRAP Monoclonal antibody (66712-1-Ig)

    IHC staining of mouse ovary using 66712-1-Ig (same clone as 66712-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 66712-1-Ig (STRAP antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66712-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using STRAP Monoclonal antibody (66712-1-Ig)

    IF Staining of HEK-293 using 66712-1-Ig (same clone as 66712-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using STRAP antibody (66712-1-Ig, Clone: 5E11F3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66712-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66712-1-PBS targets STRAP in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen STRAP fusion protein Ag13085 相同性解析による交差性が予測される生物種
Full Name serine/threonine kinase receptor associated protein
Calculated molecular weight 38 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC000162
Gene symbol STRAP
Gene ID (NCBI) 11171
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

STRAP (Serine-threonine kinase receptor-associated protein), also known as MAWD, is a ubiquitous WD40 domain protein, common function of which is to provide a suitable scaffold for coordinating multiprotein complex assemblies and thus, regulate a variety of cellular processes like signal transduction, transcriptional regulation, programmed cell death and so on. STRAP plays a role in the cellular distribution of the SMN complex, which is essential for spliceosomal snRNP assembly in the cytoplasm. STRAP inhibits transforming growth factor-beta (TGF-beta) signaling and enhances tumorigenicity via TGF-beta-dependent and -independent mechanisms. STRAP is up-regulated in several cancers and functions as an oncogene. STRAP imparts oncogenic characteristics to cells by promoting ERK and pRb phosphorylation. Moreover, STRAP regulates c-Jun stability by decreasing the ubiquitylation and proteosomal degradation of c-Jun.