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  • Featured Product
  • KD/KO Validated

STRAP Monoclonal antibody, PBS Only

STRAP Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5E11F3

Cat no : 66712-1-PBS

Synonyms

MAP activator with WD repeats, MAWD, PT WD, STRAP, UNR interacting protein, UNRIP, WD 40 repeat protein PT WD

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using STRAP Monoclonal antibody (66712-1-Ig)

    WB analysis of HEK-293 using 66712-1-Ig (same clone as 66712-1-PBS)

    WB result of STRAP antibody (66712-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STRAP transfected HEK-293 cells. This data was developed using the same antibody clone with 66712-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using STRAP Monoclonal antibody (66712-1-Ig)

    WB analysis using 66712-1-Ig (same clone as 66712-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66712-1-Ig (STRAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66712-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using STRAP Monoclonal antibody (66712-1-Ig)

    IHC staining of mouse ovary using 66712-1-Ig (same clone as 66712-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 66712-1-Ig (STRAP antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66712-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using STRAP Monoclonal antibody (66712-1-Ig)

    IHC staining of mouse ovary using 66712-1-Ig (same clone as 66712-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 66712-1-Ig (STRAP antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66712-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using STRAP Monoclonal antibody (66712-1-Ig)

    IF Staining of HEK-293 using 66712-1-Ig (same clone as 66712-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using STRAP antibody (66712-1-Ig, Clone: 5E11F3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66712-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66712-1-PBS targets STRAP in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen STRAP fusion protein Ag13085 相同性解析による交差性が予測される生物種
Full Name serine/threonine kinase receptor associated protein
Calculated molecular weight 38 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC000162
Gene symbol STRAP
Gene ID (NCBI) 11171
RRIDAB_2882063
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

STRAP (Serine-threonine kinase receptor-associated protein), also known as MAWD, is a ubiquitous WD40 domain protein, common function of which is to provide a suitable scaffold for coordinating multiprotein complex assemblies and thus, regulate a variety of cellular processes like signal transduction, transcriptional regulation, programmed cell death and so on. STRAP plays a role in the cellular distribution of the SMN complex, which is essential for spliceosomal snRNP assembly in the cytoplasm. STRAP inhibits transforming growth factor-beta (TGF-beta) signaling and enhances tumorigenicity via TGF-beta-dependent and -independent mechanisms. STRAP is up-regulated in several cancers and functions as an oncogene. STRAP imparts oncogenic characteristics to cells by promoting ERK and pRb phosphorylation. Moreover, STRAP regulates c-Jun stability by decreasing the ubiquitylation and proteosomal degradation of c-Jun.