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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STT3B Monoclonal antibody (68496-1-Ig)

    WB analysis using 68496-1-Ig (same clone as 68496-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68496-1-Ig (STT3B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68496-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using STT3B Monoclonal antibody (68496-1-Ig)

    WB analysis using 68496-1-Ig (same clone as 68496-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68496-1-Ig (STT3B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68496-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using STT3B Monoclonal antibody (68496-1-Ig)

    FC experiment of A431 using 68496-1-Ig (same clone as 68496-1-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.8 ug STT3B Monoclonal antibody (68496-1-Ig, Clone:3B7A8) and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68496-1-PBS in a different storage buffer formulation.

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STT3B Monoclonal antibody, PBS Only

STT3B Monoclonal Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 68496-1-PBS
Clone No.3B7A8

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

STT, Source of immunodominant MHC-associated peptides homolog, SIMP, EC:2.4.99.18, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68496-1-PBS targets STT3B in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen STT3B fusion protein Ag30866 相同性解析による交差性が予測される生物種
Full Name STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae)
Calculated molecular weight 97 kDa
Observed molecular weight 94 kDa
GenBank accession numberBC015880
Gene Symbol STT3B
Gene ID (NCBI) 201595
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8TCJ2
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

STT3B is a component of the N-oligosaccharyl transferase (OST) enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). SST3B seems to be involved in complex substrate specificity