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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Syntaxin 10 Polyclonal antibody (11036-1-AP)

    WB analysis of HeLa using 11036-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11036-1-AP (Syntaxin 10 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using Syntaxin 10 Polyclonal antibody (11036-1-AP)

    WB analysis of MCF-7 using 11036-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 11036-1-AP (Syntaxin 10 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of BxPC-3 cells using Syntaxin 10 Polyclonal antibody (11036-1-AP)

    WB analysis of BxPC-3 using 11036-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 11036-1-AP (Syntaxin 10 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Syntaxin 10 Polyclonal antibody (11036-1-AP)

    IHC staining of mouse heart using 11036-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11036-1-AP (Syntaxin 10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Syntaxin 10 Polyclonal antibody (11036-1-AP)

    IHC staining of mouse heart using 11036-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 11036-1-AP (Syntaxin 10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Syntaxin 10 Polyclonal antibody

Syntaxin 10 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11036-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA, IF

Synonyms

Syntaxin-10, Syn10, STX10

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, BxPC-3 cells
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11036-1-AP targets Syntaxin 10 in WB, IHC, ELISA, IF applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Syntaxin 10 fusion protein Ag1501 相同性解析による交差性が予測される生物種
Full Name syntaxin 10
Calculated molecular weight 28 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC017237
Gene Symbol Syntaxin 10
Gene ID (NCBI) 8677
RRIDAB_2198210
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60499
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Syntaxin 10 (STX10), also named as SYN10, belongs to the syntaxin family. Syntaxins are target membrane SNAREs(Soluble NSF attachment protein receptors) which are membrane components that determine the specificity of the docking and fusion of vesicles to target membranes.

Protocols

Product Specific Protocols
WB protocol for Syntaxin 10 antibody 11036-1-APDownload protocol
IHC protocol for Syntaxin 10 antibody 11036-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Eur J Immunol

SNARE protein expression and localization in human cytotoxic T lymphocytes.

Authors - Pattu Varsha V
View Article
humanWB

Front Oncol

Identification of a novel MYC target gene set signature for predicting the prognosis of osteosarcoma patients

Authors - Deliang Gong
View Article