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  • Featured Product
  • KD/KO Validated

STX17 Recombinant antibody

STX17 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

4G3

Cat no : 81899-1-RR

Synonyms

Syntaxin-17, Syntaxin 17, 4G3

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using STX17 Recombinant antibody (81899-1-RR)

    WB analysis of HepG2 using 81899-1-RR

    WB result of STX17 antibody (81899-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STX17 transfected HepG2 cells.

  • Western Blot (WB) analysis of various lysates using STX17 Recombinant antibody (81899-1-RR)

    WB analysis using 81899-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81899-1-RR (STX17 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using STX17 Recombinant antibody (81899-1-RR)

    IHC staining of mouse kidney using 81899-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 81899-1-RR (STX17 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using STX17 Recombinant antibody (81899-1-RR)

    IHC staining of mouse kidney using 81899-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 81899-1-RR (STX17 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using STX17 Recombinant antibody (81899-1-RR)

    IF Staining of HepG2 using 81899-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using STX17 antibody (81899-1-RR, Clone: 4G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Affinity and Kinetic Characterization of 81899-1-RR

    Affinity and Kinetic Characterization of 81899-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81899-1-RR against Human STX17 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inhuman testis tissue, HepG2 cells, A375 cells, HEK-293 cells, L02 cells, mouse testis issue, rat testis tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81899-1-RR targets STX17 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen STX17 fusion protein Ag12134 相同性解析による交差性が予測される生物種
Full Name syntaxin 17
Calculated molecular weight 302 aa, 33 kDa
Observed molecular weight 33-35 kD
GenBank accession numberBC111009
Gene symbol STX17
Gene ID (NCBI) 55014
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for the fusion of cellular membranes. Syntaxin 17 (STX17) is a SNARE of the autophagosome involved in autophagy through the direct control of autophagosome membrane fusion with the lysosome membrane. STX17 may also play a role in the early secretory pathway where it may maintain the architecture of the endoplasmic reticulum-Golgi intermediate compartment/ERGIC and Golgi and/or regulate transport between the endoplasmic reticulum, the ERGIC and the Golgi.

Protocols

Product Specific Protocols
WB protocol for STX17 antibody 81899-1-RRDownload protocol
IHC protocol for STX17 antibody 81899-1-RRDownload protocol
IF protocol for STX17 antibody 81899-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols