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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using STX1A Recombinant antibody (83159-6-RR)

    WB analysis using 83159-6-RR (same clone as 83159-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83159-6-RR (STX1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83159-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using STX1A Recombinant antibody (83159-6-RR)

    IHC staining of mouse brain using 83159-6-RR (same clone as 83159-6-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83159-6-RR (STX1A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83159-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using STX1A Recombinant antibody (83159-6-RR)

    IHC staining of mouse brain using 83159-6-RR (same clone as 83159-6-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83159-6-RR (STX1A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83159-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using STX1A Recombinant antibody (83159-6-RR)

    IF Staining of mouse cerebellum using 83159-6-RR (same clone as 83159-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using STX1A antibody (83159-6-RR, Clone: 230470F5 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83159-6-PBS in a different storage buffer formulation.

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STX1A Recombinant antibody, PBS Only

STX1A Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 83159-6-PBS
Clone No.230470F5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

Syntaxin 1A, STX1, p35 1, Neuron specific antigen HPC 1, HPC 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83159-6-PBS targets STX1A in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name syntaxin 1A (brain)
Calculated molecular weight 33 kDa
Observed molecular weight 33-35 kDa
GenBank accession numberNM_004603
Gene Symbol STX1A
Gene ID (NCBI) 6804
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ16623
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Syntaxins are a family of transmembrane proteins that belong to the SNARE complex (PMID: 11737951). In conjunction with other SNAREs and with the cytoplasmic NSF and SNAP proteins, syntaxins mediate vesicle fusion in diverse vesicular transport processes along the exocytic and endocytic pathways (PMID: 11737951). Syntaxin 1A (STX1A) and 1B (STX1B), two closely related isoforms of syntaxin 1, are involved in synaptic vesicle docking and fusion during neurotransmitter release (PMID: 7690687; 1321498; 18691641). This antibody recognizes human STX1A.