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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

STX1B Recombinant antibody

STX1B Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240156B9

Cat no : 83298-1-RR

Synonyms

Syntaxin 1A, Syntaxin 1B, syntaxin 1B, STX1B2, STX1B1

Validation Data Gallery

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  • Western Blot (WB) analysis of Y79 cells using STX1B Recombinant antibody (83298-1-RR)

    WB analysis of Y79 using 83298-1-RR

    Y79 cells were subjected to SDS PAGE followed by western blot with 83298-1-RR (STX1B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using STX1B Recombinant antibody (83298-1-RR)

    IHC staining of mouse eye using 83298-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 83298-1-RR (STX1B antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse eye tissue using STX1B Recombinant antibody (83298-1-RR)

    IHC staining of mouse eye using 83298-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 83298-1-RR (STX1B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using STX1B Recombinant antibody (83298-1-RR)

    IHC staining of mouse brain using 83298-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83298-1-RR (STX1B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using STX1B Recombinant antibody (83298-1-RR)

    IF Staining of mouse brain using 83298-1-RR

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using STX1B antibody (83298-1-RR, Clone: 240156B9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using STX1B Recombinant antibody (83298-1-RR)

    IF Staining of mouse brain using 83298-1-RR

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using STX1B antibody (83298-1-RR, Clone: 240156B9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of SH-SY5Y cells using STX1B Recombinant antibody (83298-1-RR)

    FC experiment of SH-SY5Y using 83298-1-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Stx1b Recombinant Antibody (83298-1-RR, Clone:240156B9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83298-1-RR

    Affinity and Kinetic Characterization of 83298-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83298-1-RR against Human STX1B were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inY79 cells
Positive IHC detected inmouse eye tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inmouse brain tissue
Positive FC (Intra) detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83298-1-RR is STX1B Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen STX1B fusion protein Ag10275 相同性解析による交差性が予測される生物種
Full Name syntaxin 1B
Calculated molecular weight 288 aa, 33 kDa
Observed molecular weight 33-35 kDa
GenBank accession numberBC062298
Gene symbol STX1B
Gene ID (NCBI) 112755
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Syntaxins are a family of transmembrane proteins that belong to the SNARE complex (PMID: 11737951). In conjunction with other SNAREs and with the cytoplasmic NSF and SNAP proteins, syntaxins mediate vesicle fusion in diverse vesicular transport processes along the exocytic and endocytic pathways (PMID: 11737951). Syntaxin 1A and 1B, two closely related isoforms of syntaxin 1, are involved in synaptic vesicle docking and fusion during neurotransmitter release (PMID: 7690687; 1321498; 18691641).

Protocols

Product Specific Protocols
WB protocol for STX1B antibody 83298-1-RRDownload protocol
IHC protocol for STX1B antibody 83298-1-RRDownload protocol
IF protocol for STX1B antibody 83298-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols