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- KD/KO Validated
Syntaxin 2 Polyclonal antibody
Syntaxin 2 Polyclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 55033-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells, HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 5 publications below |
| IHC | See 3 publications below |
| IF | See 1 publications below |
Product Information
55033-1-AP targets Syntaxin 2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | syntaxin 2 |
| Calculated molecular weight | 33 kDa |
| Observed molecular weight | 32 kDa |
| GenBank accession number | NM_001980 |
| Gene symbol | Syntaxin 2 |
| Gene ID (NCBI) | 2054 |
| RRID | AB_11182166 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | 0.1M NaHCO3, 0.1M glycine, 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
Syntaxin 2 (STX2), also named as EPIM, STX2A, STX2B and STX2C, belongs to the syntaxin family. It is essential for epithelial morphogenesis. STX2 is a member of the SNARE membrane fusion machinery which localized to the midbody during cytokinesis in rat and canine kidney cell lines. Inhibition of STX2 and Vamp8 function by overexpression of nonmembrane-anchored mutants caused failure of cytokinesis leading to the formation of binucleated cells. Time-lapse microscopy showed that only midbody abscission and not further upstream events, such as furrowing, were affected. The antibody is specific to STX2.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Syntaxin 2 antibody 55033-1-AP | Download protocol |
| IF protocol for Syntaxin 2 antibody 55033-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Death Dis STX2 promotes colorectal cancer metastasis through a positive feedback loop that activates the NF-κB pathway.
| ||
J Cell Sci Syntaxin 7 contributes to breast cancer cell invasion by promoting invadopodia formation. | ||
Life Sci STX2 drives colorectal cancer proliferation via upregulation of EXOSC4.
| ||
J Cancer Syntaxin 2 promotes colorectal cancer growth by increasing the secretion of exosomes. | ||
Neuroscience SNAP25/syntaxin4/VAMP2/Munc18-1 Complexes in Spinal Dorsal Horn Contributed to Inflammatory Pain. |
