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KD/KO Validated

Syntaxin 4 Polyclonal antibody

Name: Syntaxin 4 Polyclonal antibody
Brand: Proteintech
SKU: 14988-1-AP

Syntaxin 4 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 14988-1-AP

Synonyms

Syntaxin-4, STX4A, STX4, Renal carcinoma antigen NY-REN-31

Validation Data Gallery

Filter:

AllWBIPIHC

WB analysis using 14988-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14988-1-AP (Syntaxin 4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 14988-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 14988-1-AP (Syntaxin 4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 14988-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 14988-1-AP (Syntaxin 4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of rat kidney using 14988-1-AP
rat kidney tissue were subjected to SDS PAGE followed by western blot with 14988-1-AP (Syntaxin 4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 14988-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14988-1-AP (Syntaxin 4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IP experiment of mouse kidney using 14988-1-AP
IP result of anti-Syntaxin 4 (IP:14988-1-AP, 4ug; Detection:14988-1-AP 1:1500) with mouse kidney tissue lysate 4000ug.
IHC staining of human stomach using 14988-1-AP
Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 14988-1-AP (Syntaxin 4 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 14988-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse kidney tissue, rat kidney tissue, HEK-293 cells, Jurkat cells
Positive IP detected in	mouse kidney tissue
Positive IHC detected in	human stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 8 publications below

Product Information

14988-1-AP targets Syntaxin 4 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Syntaxin 4 fusion protein Ag6973 相同性解析による交差性が予測される生物種
Full Name	syntaxin 4
Calculated molecular weight	34 kDa
Observed molecular weight	35-37 kDa
GenBank accession number	BC002436
Gene symbol	STX4
Gene ID (NCBI)	6810
RRID	AB_2286910
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for Syntaxin 4 antibody 14988-1-AP	Download protocol
IHC protocol for Syntaxin 4 antibody 14988-1-AP	Download protocol
IP protocol for Syntaxin 4 antibody 14988-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Brain Reduced oligodendrocyte exosome secretion in multiple system atrophy involves SNARE dysfunction. Authors - Zhenwei Yu View Article
human	WB	Front Immunol Bi-Allelic Mutations in STXBP2 Reveal a Complementary Role for STXBP1 in Cytotoxic Lymphocyte Killing. Authors - Jamie A Lopez View Article
human	WB	Int J Biol Macromol PKCε controls the fusion of secretory vesicles in mast cells in a phosphatidic acid-dependent mode. Authors - Emilio M Serrano-López View Article
human	WB	Br J Pharmacol CRP promotes atherosclerosis by increasing LDL transcytosis across endothelial cells. Authors - Fang Bian View Article
human	WB	J Biol Chem Glia Maturation Factor-γ Regulates Monocyte Migration through Modulation of β1-Integrin. Authors - Wulin Aerbajinai View Article
human	WB	J Transl Med Angiopoietin2-mediated caveolin1 phosphorylation regulating transcytosis of renal tubular epithelial cell contributes to the occurrence of albuminuria under high glucose exposure. Authors - Jing Liu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Syntaxin 4 Polyclonal antibody

Syntaxin 4 Polyclonal Antibody for WB, IHC, IP, ELISA

Validation Data Gallery

WB analysis using 14988-1-AP

WB analysis of Jurkat using 14988-1-AP

WB analysis of HEK-293 using 14988-1-AP

WB analysis of rat kidney using 14988-1-AP

WB analysis of mouse kidney using 14988-1-AP

IP experiment of mouse kidney using 14988-1-AP

IHC staining of human stomach using 14988-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Reduced oligodendrocyte exosome secretion in multiple system atrophy involves SNARE dysfunction.

Bi-Allelic Mutations in STXBP2 Reveal a Complementary Role for STXBP1 in Cytotoxic Lymphocyte Killing.

PKCε controls the fusion of secretory vesicles in mast cells in a phosphatidic acid-dependent mode.

CRP promotes atherosclerosis by increasing LDL transcytosis across endothelial cells.

Glia Maturation Factor-γ Regulates Monocyte Migration through Modulation of β1-Integrin.

Angiopoietin2-mediated caveolin1 phosphorylation regulating transcytosis of renal tubular epithelial cell contributes to the occurrence of albuminuria under high glucose exposure.