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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

STX5 Polyclonal antibody

STX5 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 26711-1-AP

Synonyms

SED5, STX5, STX5A, syntaxin 5

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using STX5 Polyclonal antibody (26711-1-AP)

    WB analysis using 26711-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26711-1-AP (STX5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using STX5 Polyclonal antibody (26711-1-AP)

    IHC staining of mouse brain using 26711-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26711-1-AP (STX5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using STX5 Polyclonal antibody (26711-1-AP)

    IHC staining of mouse brain using 26711-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26711-1-AP (STX5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using STX5 Polyclonal antibody (26711-1-AP)

    IHC staining of mouse brain using 26711-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26711-1-AP (STX5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using STX5 Polyclonal antibody (26711-1-AP)

    IHC staining of mouse brain using 26711-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26711-1-AP (STX5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using STX5 Polyclonal antibody (26711-1-AP)

    IF Staining of mouse brain using 26711-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 26711-1-AP (STX5 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using STX5 Polyclonal antibody (26711-1-AP)

    IF Staining of mouse brain using 26711-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 26711-1-AP (STX5 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inA431 cells, HEK-293 cells, HepG2 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

26711-1-AP targets STX5 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen STX5 fusion protein Ag25098 相同性解析による交差性が予測される生物種
Full Name syntaxin 5
Calculated molecular weight 32 kDa, 34 kDa, 42 kDa
Observed molecular weight 35 and 42 kDa
GenBank accession numberBC012137
Gene symbol STX5
Gene ID (NCBI) 6811
RRIDAB_2880609
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for STX5 antibody 26711-1-APDownload protocol
IHC protocol for STX5 antibody 26711-1-APDownload protocol
IF protocol for STX5 antibody 26711-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Biol Chem

Competition for cysteine acylation by C16:0 and C18:0 derived lipids is a global phenomenon in the proteome

Authors - Hana Nůsková
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Wei (Verified Customer) (06-29-2020)

Double band for whole heart tissues. The upper band is much dense than the lower band.

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse Heart