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  • Featured Product
  • KD/KO Validated

Syntaxin 6 Polyclonal antibody

Syntaxin 6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 10841-1-AP

Synonyms

Syntaxin-6, STX6

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using Syntaxin 6 Polyclonal antibody (10841-1-AP)

    WB analysis of A549 using 10841-1-AP

    WB result of Syntaxin 6 antibody (10841-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Syntaxin 6 transfected A549 cells.

  • Western Blot (WB) analysis of various lysates using Syntaxin 6 Polyclonal antibody (10841-1-AP)

    WB analysis using 10841-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10841-1-AP (Syntaxin 6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using Syntaxin 6 Polyclonal antibody (10841-1-AP)

    WB analysis of human brain using 10841-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 10841-1-AP (Syntaxin 6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using Syntaxin 6 Polyclonal antibody (10841-1-AP)

    WB analysis of mouse brain using 10841-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10841-1-AP (Syntaxin 6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using Syntaxin 6 Polyclonal antibody (10841-1-AP)

    WB analysis of rat brain using 10841-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 10841-1-AP (Syntaxin 6 antibody at dilution of 1:2000 incubated at 4 degree celsius over night.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using Syntaxin 6 Polyclonal antibody (10841-1-AP)

    IP experiment of mouse brain using 10841-1-AP

    IP result of anti-Syntaxin 6 (IP:10841-1-AP, 3ug; Detection:10841-1-AP 1:1000) with mouse brain tissue lysate 7000ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Syntaxin 6 Polyclonal antibody (10841-1-AP)

    IHC staining of mouse brain using 10841-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10841-1-AP (Syntaxin 6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Syntaxin 6 Polyclonal antibody (10841-1-AP)

    IHC staining of mouse brain using 10841-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10841-1-AP (Syntaxin 6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Syntaxin 6 Polyclonal antibody (10841-1-AP)

    IHC staining of human prostate cancer using 10841-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10841-1-AP (Syntaxin 6 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Syntaxin 6 Polyclonal antibody (10841-1-AP)

    IHC staining of human prostate cancer using 10841-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10841-1-AP (Syntaxin 6 antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Syntaxin 6 Polyclonal antibody (10841-1-AP)

    IHC staining of human prostate cancer using 10841-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10841-1-AP (Syntaxin 6 antibody at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using Syntaxin 6 Polyclonal antibody (10841-1-AP)

    IF Staining of PC-12 using 10841-1-AP

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using Syntaxin 6 antibody (10841-1-AP) at dilution of 1:700 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using Syntaxin 6 Polyclonal antibody (10841-1-AP)

    IF Staining of PC-12 using 10841-1-AP

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using 66477-1-Ig (TGN46 antibody) at dilution of 1:100 and 10841-1-AP (Syntaxin 6 antibody) at dilution of 1:50, further stained with CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L) for 66477-1-Ig, and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L) for 10841-1-AP.

  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using Syntaxin 6 Polyclonal antibody (10841-1-AP)

    IF Staining of PC-12 using 10841-1-AP

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using Syntaxin 6 antibody (10841-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of murine BM-DC using Syntaxin 6 Polyclonal antibody (10841-1-AP)

    IF Staining of murine BM-DC using 10841-1-AP

    IF result of anti-STX6 (10841-1-AP) in Murine BM-DC by Dr. LOREDANA SAVEANU.

  • Immunofluorescence (IF) / fluorescent staining of murine Spleen-DC using Syntaxin 6 Polyclonal antibody (10841-1-AP)

    IF Staining of murine Spleen-DC using 10841-1-AP

    IF result of anti-STX6 (10841-1-AP) in Murine Spleen-DC by Dr. LOREDANA SAVEANU.

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Tested Applications

Positive WB detected inNeuro-2a cells, human brain tissue, mouse brain tissue, rat brain tissue, A549 cells, PC-12 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-12 cells, murine BM-DC, murine Spleen-DC

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:350-1:1400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10841-1-AP targets Syntaxin 6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Syntaxin 6 fusion protein Ag1284 相同性解析による交差性が予測される生物種
Full Name syntaxin 6
Calculated molecular weight 29 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC009944
Gene symbol Syntaxin 6
Gene ID (NCBI) 10228
RRIDAB_2196506
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Syntaxin-6 (STX6) is a SNARE protein located in the trans-Golgi network (TGN) and endosomes. It associates with a variety of SNARE proteins and is involved in intracellular vesicle trafficking. STX6 is required for sorting proteins from endosomes toward either the TGN or lysosomes.

Protocols

Product Specific Protocols
WB protocol for Syntaxin 6 antibody 10841-1-APDownload protocol
IHC protocol for Syntaxin 6 antibody 10841-1-APDownload protocol
IF protocol for Syntaxin 6 antibody 10841-1-APDownload protocol
IP protocol for Syntaxin 6 antibody 10841-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Science

IRAP identifies an endosomal compartment required for MHC class I cross-presentation.

Authors - Loredana Saveanu
View Article
mouseIF

Nat Immunol

IRAP(+) endosomes restrict TLR9 activation and signaling.

Authors - Joel Babdor
View Article
humanWB

Cell Rep Med

Compromised mitochondrial quality control triggers lipin1-related rhabdomyolysis.

Authors - Yamina Hamel
View Article
IF,FC

Nat Commun

IRAP-dependent endosomal T cell receptor signalling is essential for T cell responses.

Authors - Irini Evnouchidou
View Article
mouseIF

Cell Rep

Innate Immune Signals Induce Anterograde Endosome Transport Promoting MHC Class I Cross-Presentation.

Authors - Mirjana Weimershaus
View Article
mouseIF

J Immunol

Conventional dendritic cells require IRAP-Rab14 endosomes for efficient cross-presentation.

Authors - Weimershaus Mirjana M
View Article