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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Syntaxin 6 Recombinant antibody (84627-5-RR)

    WB analysis using 84627-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84627-5-RR (Syntaxin 6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Syntaxin 6 Recombinant antibody (84627-5-RR)

    WB analysis using 84627-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84627-5-RR (Syntaxin 6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using Syntaxin 6 Recombinant antibody (84627-5-RR)

    IP experiment of mouse brain using 84627-5-RR

    IP result of anti-Syntaxin 6 (IP:84627-5-RR, 4ug; Detection:84627-5-RR 1:1500) with mouse brain tissue lysate 1840 ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Syntaxin 6 Recombinant antibody (84627-5-RR)

    IHC staining of mouse brain using 84627-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84627-5-RR (Syntaxin 6 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using Syntaxin 6 Recombinant antibody (84627-5-RR)

    IF Staining of PC-12 using 84627-5-RR

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using Syntaxin 6 antibody (84627-5-RR, Clone: 242100A9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84627-5-RR

    Affinity and Kinetic Characterization of 84627-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84627-5-RR against Human Syntaxin 6 were performed. The affinity constant is 1.55 nM.

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Syntaxin 6 Recombinant antibody

Syntaxin 6 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 84627-5-RR
Clone No.242100A9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Syntaxin-6, STX6, 242100A9

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, PC-12 cells, Neuro-2a cells, fetal human brain tissue, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84627-5-RR targets Syntaxin 6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Syntaxin 6 fusion protein Ag1284 相同性解析による交差性が予測される生物種
Full Name syntaxin 6
Calculated molecular weight 29 kDa
Observed molecular weight32 kDa
GenBank accession numberBC009944
Gene Symbol Syntaxin 6
Gene ID (NCBI) 10228
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO43752
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Syntaxin-6 (STX6) is a SNARE protein located in the trans-Golgi network (TGN) and endosomes. It associates with a variety of SNARE proteins and is involved in intracellular vesicle trafficking. STX6 is required for sorting proteins from endosomes toward either the TGN or lysosomes.

Protocols

Product Specific Protocols
WB protocol for Syntaxin 6 antibody 84627-5-RRDownload protocol
IHC protocol for Syntaxin 6 antibody 84627-5-RRDownload protocol
IF protocol for Syntaxin 6 antibody 84627-5-RRDownload protocol
IP protocol for Syntaxin 6 antibody 84627-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols