Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of MDCK cells using 12322-1-AP
siRNA result of 12322-1-AP (anti-STX7) by Dr.Kazunari Yamashita.
WB analysis of mouse spleen using 12322-1-AP
mouse spleen tissue were subjected to SDS PAGE followed by western blot with 12322-1-AP (Syntaxin 7 antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 12322-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12322-1-AP (Syntaxin 7 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 12322-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 12322-1-AP (Syntaxin 7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 12322-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 12322-1-AP (Syntaxin 7 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse spleen using 12322-1-AP
mouse spleen tissue were subjected to SDS PAGE followed by western blot with 12322-1-AP (Syntaxin 7 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IP experiment of mouse heart using 12322-1-AP
IP result of anti-Syntaxin 7 (IP:12322-1-AP, 3ug; Detection:12322-1-AP 1:800) with mouse heart tissue lysate 4000ug.
IHC staining of human medulloblastoma using 12322-1-AP
Immunohistochemical analysis of paraffin-embedded human medulloblastoma tissue slide using 12322-1-AP (Syntaxin 7 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human medulloblastoma using 12322-1-AP
Immunohistochemical analysis of paraffin-embedded human medulloblastoma tissue slide using 12322-1-AP (Syntaxin 7 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A549 using 12322-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using Syntaxin 7 antibody (12322-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Featured Product
KD/KO Validated

Syntaxin 7 Polyclonal antibody

Syntaxin 7 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12322-1-AP

Publications(12)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, canine and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

Syntaxin-7, STX7

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (12)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse spleen tissue, MDCK cells
Positive IP detected in	mouse heart tissue
Positive IHC detected in	human medulloblastoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A549 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 8 publications below
IHC	See 1 publications below
IF	See 2 publications below
CoIP	See 2 publications below

Product Information

12322-1-AP targets Syntaxin 7 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, canine samples.

Tested Reactivity	human, mouse, canine
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Syntaxin 7 fusion protein Ag2975 相同性解析による交差性が予測される生物種
Full Name	syntaxin 7
Calculated molecular weight	261 aa, 30 kDa
Observed molecular weight	35-40 kDa
GenBank accession number	BC011975
Gene Symbol	Syntaxin 7
Gene ID (NCBI)	8417
RRID	AB_2239979
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O15400
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for Syntaxin 7 antibody 12322-1-AP	Download protocol
IHC protocol for Syntaxin 7 antibody 12322-1-AP	Download protocol
IF protocol for Syntaxin 7 antibody 12322-1-AP	Download protocol
IP protocol for Syntaxin 7 antibody 12322-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	WB	Proc Natl Acad Sci U S A Crucial role of the hydrophobic pocket region of Munc18 protein in mast cell degranulation. Authors - Bin Na-Ryum NR View Article
rat	WB,CoIP	J Cell Biol VAMP4 regulates insulin levels by targeting secretory granules to lysosomes Authors - Min Li KD Validated View Article
human	WB	Biochem J Phosphoproteomics reveals that the hVPS34 regulated SGK3 kinase specifically phosphorylates endosomal proteins including Syntaxin-7, Syntaxin-12, RFIP4 and WDR44. Authors - Nazma Malik View Article
human	WB	Cell Logist Interaction with the effector dynamin-related protein 1 (Drp1) is an ancient function of Rab32 subfamily proteins. Authors - Carolina G Ortiz-Sandoval View Article
human,mouse	WB,IF	Neurobiol Dis Syntaxin 7 modulates seizure activity in epilepsy Authors - Junhong Wu View Article
human	WB	J Biol Chem Competition for cysteine acylation by C16:0 and C18:0 derived lipids is a global phenomenon in the proteome Authors - Hana Nůsková View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of MDCK cells using 12322-1-AP

WB analysis of mouse spleen using 12322-1-AP

WB analysis of mouse thymus using 12322-1-AP

WB analysis of human liver using 12322-1-AP

WB analysis of mouse liver using 12322-1-AP

WB analysis of mouse spleen using 12322-1-AP

IP experiment of mouse heart using 12322-1-AP

IHC staining of human medulloblastoma using 12322-1-AP

IHC staining of human medulloblastoma using 12322-1-AP

IF Staining of A549 using 12322-1-AP

Syntaxin 7 Polyclonal antibody

Syntaxin 7 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Crucial role of the hydrophobic pocket region of Munc18 protein in mast cell degranulation.

VAMP4 regulates insulin levels by targeting secretory granules to lysosomes

Phosphoproteomics reveals that the hVPS34 regulated SGK3 kinase specifically phosphorylates endosomal proteins including Syntaxin-7, Syntaxin-12, RFIP4 and WDR44.

Interaction with the effector dynamin-related protein 1 (Drp1) is an ancient function of Rab32 subfamily proteins.

Syntaxin 7 modulates seizure activity in epilepsy

Competition for cysteine acylation by C16:0 and C18:0 derived lipids is a global phenomenon in the proteome