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STXBP1 Polyclonal antibody

Name: STXBP1 Polyclonal antibody
Brand: Proteintech
SKU: 11459-1-AP

STXBP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11459-1-AP

Synonyms

N-Sec1, N Sec1, Munc18-1, MUNC18 1, FLJ37475

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 11459-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11459-1-AP (STXBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11459-1-AP
WB result of STXBP1 antibody (11459-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STXBP1 transfected HeLa cells.
WB analysis of A549 using 11459-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 11459-1-AP (STXBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A375 using 11459-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 11459-1-AP (STXBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Y79 using 11459-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 11459-1-AP (STXBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11459-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11459-1-AP (STXBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 11459-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11459-1-AP (STXBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 11459-1-AP
IP result of anti-STXBP1 (IP:11459-1-AP, 4ug; Detection:11459-1-AP 1:1000) with HeLa cells lysate 2400ug.
IHC staining of human brain using 11459-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11459-1-AP (STXBP1 antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human brain using 11459-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11459-1-AP (STXBP1 antibody) at dilution of 1:200 (under 40x lens).
IF Staining of A375 using 11459-1-AP
Immunofluorescent analysis of A375 cells using 11459-1-AP (STXBP1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 11459-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, A549 cells, A375 cells, human brain tissue, Y79 cells, Jurkat cells, SH-SY5Y cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A375 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 13 publications below
IHC	See 3 publications below
IF	See 2 publications below

Product Information

11459-1-AP targets STXBP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	STXBP1 fusion protein Ag1978 相同性解析による交差性が予測される生物種
Full Name	syntaxin binding protein 1
Calculated molecular weight	594 aa, 68 kDa
Observed molecular weight	65-68 kDa
GenBank accession number	BC015749
Gene symbol	STXBP1
Gene ID (NCBI)	6812
RRID	AB_2196690
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for STXBP1 antibody 11459-1-AP	Download protocol
IHC protocol for STXBP1 antibody 11459-1-AP	Download protocol
IF protocol for STXBP1 antibody 11459-1-AP	Download protocol
IP protocol for STXBP1 antibody 11459-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	WB, IHC	EMBO J Munc13 activates the Munc18-1/syntaxin-1 complex and enables Munc18-1 to prime SNARE assembly. Authors - Xianping Wang View Article
mouse	WB, IF	Proc Natl Acad Sci U S A Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome. Authors - Poppy Datta View Article
mouse	WB	J Biol Chem The myosin-tail homology domain of centrosomal protein 290 is essential for protein confinement between the inner and outer segments in photoreceptors. Authors - Poppy Datta View Article
rat	WB	Neuroscience SNAP25/syntaxin4/VAMP2/Munc18-1 Complexes in Spinal Dorsal Horn Contributed to Inflammatory Pain. Authors - Xing-Lian Duan View Article
mouse	WB, IHC	PLoS One Differential requirement of NPHP1 for compartmentalized protein localization during photoreceptor outer segment development and maintenance. Authors - Poppy Datta View Article
mouse	WB	Cell Rep Neuron-specific protein network mapping of autism risk genes identifies shared biological mechanisms and disease-relevant pathologies Authors - Nadeem Murtaza View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

STXBP1 Polyclonal antibody

STXBP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 11459-1-AP

WB analysis of HeLa using 11459-1-AP

WB analysis of A549 using 11459-1-AP

WB analysis of A375 using 11459-1-AP

WB analysis of Y79 using 11459-1-AP

WB analysis of HeLa using 11459-1-AP

WB analysis of human brain using 11459-1-AP

IP experiment of HeLa using 11459-1-AP

IHC staining of human brain using 11459-1-AP

IHC staining of human brain using 11459-1-AP

IF Staining of A375 using 11459-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Munc13 activates the Munc18-1/syntaxin-1 complex and enables Munc18-1 to prime SNARE assembly.

Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome.

The myosin-tail homology domain of centrosomal protein 290 is essential for protein confinement between the inner and outer segments in photoreceptors.

SNAP25/syntaxin4/VAMP2/Munc18-1 Complexes in Spinal Dorsal Horn Contributed to Inflammatory Pain.

Differential requirement of NPHP1 for compartmentalized protein localization during photoreceptor outer segment development and maintenance.

Neuron-specific protein network mapping of autism risk genes identifies shared biological mechanisms and disease-relevant pathologies